The Ideal 3D Cell Culture Model
2D cell culture is not a true reflection of the physiological cell environment. In a real body, cells grow in 3D, connecting to other cells and the extracellular matrix (ECM) to build tissues and organs. Therefore, 3D cell culture can better mimic the original tissue’s specific characteristics. Some of the processes studied in 2D culture such as gene expression, apoptosis, and, importantly, drug uptake and toxicity may not be directly transferable to in vivo experiments.
BIOMIMESYS® is a Hyaluronic acid (HA) based scaffold 3D cell culture model. This groundbreaking 3D cell culture technology associates the behavior of a solid scaffold and of a hydrogel, which we call “Hydroscaffold”. The Hydroscaffold along with its patented technology can mimic different cell’s extra-cellular matrix (ECM).
The Importance of Extra-Cellular Matrix (ECM)
The ECM’s important functions are:
- Maintain the structure of tissues and organs
- Define microenvironment for cells
- Determine cell behavior and function
These functions allow cells to anchor and migrate, communicate with each other, and differentiate and proliferate. ECM composition is organ specific. It also dynamically changes through development and function (healthy – diseased).
Side Note: Components of ECM
- Hyaluronic Acid
- Maintains the structure of the tisse
- Blinds to different growth factors and absorb water
- Key to cell-matrix interaction
- Hyaluronic Acid
- Structural Proteins
- These proteins, such as nidogen and entactin, are the “glue” that hold the ECM together
BIOMIMESYS® the Best Alternative
The ideal ECM for research is decellularized organs, but the cost, reproducibility, and scalability make it impractical for continuous use. The following characteristics of the ECM that BIOMIMESYS® provides is the best alternative.
- Hyaluronic acid grafted with adhesion proteins and collagens, elements naturally present in ECM
- Possesses a similar structure in hydrated stage to natural organs
2. Mechanical Properties
- The porous nature of the scaffold provides greater diffusion of oxygen and nutrients for better viability and greatly decreases the formation of necrotic core, often seen in other spherical 3D models.
- The abundance of metabolic enzyme activities and normal cellular polarity makes it ideal for in vitro models
- Mechanical stress (stiffness)
- The stiffness of the ECM differs from organ to organ, furthermore, differs from healthy to diseased.
- BIOMIMESYS® scaffold can be customized to have an elastic modulus of up to 20kPa, whereas with Matrigel the maximum is roughly 0.9kPa.
3. Long Term Viability
The long-term viability of cells is an important factor in cell culture, especially in drug discovery. Cells cultured with BIOMIMESYS® has been tested to have a viable for up to 28 days.
Proof of concept: Increased Culture Time [PDF]
4. Compatible With Most Downstream Applications
BIOMIMESYS® hydroscaffold has many properties (transparent, porous, biodegradable, & solid) making it ideal for use with numerous downstream applications.
5. Ready to Use
Unlike other models, such as Matrigel, BIOMIMESYS® is ready to use and requires minimal prep time. There is no thawing process, aliquoting, mixing, etc. Just open the packaging, add cell suspension, and add culture media.
Ready-to-use for immediate cell seeding. No preparation required.
Long prep time equals to increase chance of errors,
and waste of resource and time leading to increase in running cost.
Comparison with other models
What does the BIOMIMESYS® Hydroscaffold look like?
Ready to use models
** BIOMIMESYS® composition and mechanical properties (stiffness and porosity) is customizable**
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FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES