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    BIOMIMESYS®

    New generation of mimetic hydroscaffold for 3D cell culture

    Product Basics

    The Ideal 3D Cell Culture Model

    2D cell culture is not a true reflection of the physiological cell environment. In a real body, cells grow in 3D, connecting to other cells and the extracellular matrix (ECM) to build tissues and organs. Therefore, 3D cell culture can better mimic the original tissue’s specific characteristics. Some of the processes studied in 2D culture such as gene expression, apoptosis, and, importantly, drug uptake and toxicity may not be directly transferable to in vivo experiments.

    BIOMIMESYS® is a Hyaluronic acid (HA) based scaffold 3D cell culture model. This groundbreaking 3D cell culture technology associates the behavior of a solid scaffold and of a hydrogel, which we call “Hydroscaffold”. The Hydroscaffold along with its patented technology can mimic different cell’s extra-cellular matrix (ECM).

    Key Features

    Technical Information

    The Importance of Extra-Cellular Matrix (ECM)

    The ECM’s important functions are:

    • Maintain the structure of tissues and organs
    • Define microenvironment for cells
    • Determine cell behavior and function

    These functions allow cells to anchor and migrate, communicate with each other, and differentiate and proliferate. ECM composition is organ specific. It also dynamically changes through development and function (healthy – diseased).

    Side Note: Components of ECM

    Structural Elements

    • Glycosaminoglycans(GAG)
      • Hyaluronic Acid
        • Maintains the structure of the tisse
        • Blinds to different growth factors and absorb water
        • Key to cell-matrix interaction
      • Proteoglycans
    • Structural Proteins
      • Collagen
      • Elastin
    Adhesion Proteins
    • Fibronectin
    • Laminin
    Linker proteins
    • These proteins, such as nidogen and entactin, are the “glue” that hold the ECM together

    BIOMIMESYS® the Best Alternative

    The ideal ECM for research is decellularized organs, but the cost, reproducibility, and scalability make it impractical for continuous use. The following characteristics of the ECM that BIOMIMESYS® provides is the best alternative.

    1. Composition

    • Hyaluronic acid grafted with adhesion proteins and collagens, elements naturally present in ECM
    • Possesses a similar structure in hydrated stage to natural organs

    2. Mechanical Properties

    • Porosity
      • The porous nature of the scaffold provides greater diffusion of oxygen and nutrients for better viability and greatly decreases the formation of necrotic core, often seen in other spherical 3D models.
      • The abundance of metabolic enzyme activities and normal cellular polarity makes it ideal for in vitro models
    • Mechanical stress (stiffness)
      • The stiffness of the ECM differs from organ to organ, furthermore, differs from healthy to diseased.
      • BIOMIMESYS® scaffold can be customized to have an elastic modulus of up to 20kPa, whereas with Matrigel the maximum is roughly 0.9kPa.

    3. Long Term Viability

    The long-term viability of cells is an important factor in cell culture, especially in drug discovery.  Cells cultured with BIOMIMESYS® has been tested to have a viable for up to 28 days.
    Proof of concept: Increased Culture Time [PDF]

    4. Compatible With Most Downstream Applications

    BIOMIMESYS® hydroscaffold has many properties (transparent, porous, biodegradable, & solid) making it ideal for use with numerous downstream applications. 

    5. Ready to Use

    Unlike other models, such as Matrigel, BIOMIMESYS® is ready to use and requires minimal prep time. There is no thawing process, aliquoting, mixing, etc. Just open the packaging, add cell suspension, and add culture media.

    Ready-to-use for immediate cell seeding. No preparation required.

    Long prep time equals to increase chance of errors,
    and waste of resource 
    and time leading to increase in running cost.

    Comparison with other models

    What does the BIOMIMESYS® Hydroscaffold look like?

    BIOMIMESYS Hydroscaffold within 96 well plate.

    * Size is customizable

    Do not remove the hydroscaffold before use

    Translucent after seeding

    Ready to use models

    ** BIOMIMESYS® composition and mechanical properties (stiffness and porosity) is customizable**

    BIOMIMESYS® Series

    3D cell culture model for tumor cells

    3D cell culture model for adipose tissue

    3D cell culture model for Liver

    3D cell culture model for Brain

    Related Resources

    References

    1.Lu, P., Weaver, V. M. & Werb, Z. The extracellular matrix: A dynamic niche in cancer progression. J Cell Biol 196, 395 (2012).

    2.Kola, I. & Landis, J. Can the pharmaceutical industry reduce attrition rates? Nat Rev Drug Discov 3, 711–715 (2004).

    3.Wang, L., Johnson, J. A., Zhang, Q. & Beahm, E. K. Combining decellularized human adipose tissue extracellular matrix and adipose-derived stem cells for adipose tissue engineering. Acta Biomater 9, 8921–8931 (2013).Mazza, 2015

    4.Mazza, G. et al. Decellularized human liver as a natural 3D-scaffold for liver bioengineering and transplantation. Sci Rep 5, 13079 (2015).

    5.Lodish, H. et al. Integrating Cells Into Tissues. in Molecular Cell Biology (Freeman, W. H. & Company, 2003).

    6.Peach, R. J., Hollenbaugh, D., Stamenkovic, I. & Aruffo, A. Identification of hyaluronic acid binding sites in the extracellular domain of CD44. J Cell Biol 122, 257–264 (1993).

    7. Colom, A. et al. Oxygen diffusion and consumption in extracellular matrix gels: implications for designing three-dimensional cultures. J Biomed Mater Res A 102, 2776–2784 (2014).

    8.McMurtrey, R. J. Analytic Models of Oxygen and Nutrient Diffusion, Metabolism Dynamics, and Architecture Optimization in Three-Dimensional Tissue Constructs with Applications and Insights in Cerebral Organoids. Tissue engineering. Part C, Methods 22, 221–49 (2016).

    9. Al-Ani, A. et al. Oxygenation in cell culture: Critical parameters for reproducibility are routinely not reported. PLoS One 13, e0204269 (2018)

    FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES