BIOMIMESYS® Adipose Tissue is a Hyaluronic acid (HA) based scaffold 3D cell culture model. This groundbreaking 3D cell culture technology associates the behavior of a solid scaffold and of a hydrogel, which we call “Hydroscaffold”. The Hydroscaffold’s composition and mechanical properties can mimics the microenvironment of adipose tissue. It is proven to provide better in vitro/in vivo correlation and cell longevity than 2D models.
Mimics the microenvironment of adipose tissue
BIOMIMESYS® Adipose Tissue composition and organization are similar to in vivo adipose tissue by including collagens I, VI and fibronectin (RGDS pattern) in a cross-linked hyaluronic acid gel.
In order to demonstrate the induction of adipogenesis by the HCS Pharma hydroscaffold, BIOMIMESYS® Adipose tissue, various tests were performed on two cell types: 3T3-L1 and Human White Preadipocyte (HWP) according to the following culture protocol.
Protocol: as in 2D culture, seeding and differentiation steps are induced from day 0 (for HWP and 3T3-L1) until complete maturation.
Figure: A and B: Adipocyte schematic morphology in vivo.
C and D: Light-microscopy images of adipocyte differentiated from preadipocyte HWP (Human white preadipocytes) at 7 days after seeded. HWP cultured in 2D exhibited a fibroblastic appearance (C), whereas in BIOMIMESYS® Adipocyte, it was resembling morphology of mature adipocytes in vivo, showing the establishment of their principal function, fat accumulation, resulting in the presence of vesicles (D).
E and F : SEM images of 3T3-L1 (murine preadipocytes) at day 7 (E) and HWP at day 14 (F), both were cultured in BIOMIMESYS® Adipocyte. In the hydroscaffold, adipocytes formed aggregates which increased in size over time.
Better differentiation and cell longevity
BIOMIMESYS® Adipose Tissue is dedicated to the culture of adipocytes: compared to 2D culture, primary human pre-adipocytes in BIOMIMESYS® Adipose Tissue (3D) showed optimal differentiation into adipocytes, and their functionality (such as lipid storage in a single large vacuole) was maintained until 28 days.
For a better in vitro/in vivo correlation
Figure 2: Lipid accumulation in 3T3-L1 with or with Retinoic acid, compared to 2D cultures (n=3, two-ways ANOVA statistics with Tukey post-hoc ; * : p<0,05 ; ** : p<0,01 ; *** : p<0,001)
To quantify the inhibition of lipogenesis, human pre-adipocytes and 3T3-L1 cells cultured in BIOMIMESYS® Adipose tissue and in 2D culture are treated with Retinoic acid, a well-known regulator. Lipid accumulation is measured using the AdipoRed ™ kit, normalized by DNA level.
As low as 1μM of Retinoic acid led to a significant lipids accumulation inhibition in 2D, whereas at least 5μM of this molecule was needed to significantly inhibit the lipogenesis of adipocytes grown in BIOMIMESYS® Adipose tissue.
Ready to Use
BIOMIMESYS® Adipose Tissue is EASY & READY TO USE. Upon receiving the vacuum sealed 96-well plate open it (under a hood) and add the cells directly on top of the matrix. Changing the culture medium is easy as well. To remove medium, simply draw the medium with a pipette between the matrix and the edge of the well. To refresh the medium, place fresh medium onto the surface of the matrix.
Successfully Tested Cells
Human breast adenocarcinoma : MCF-7
Human breast carcinoma : CAL-51 / T-47D / MDAMB-231 / MCF10A
Normal mouse preadipocytes : 3T3-L1 / 3T3-F442A
Human colorectal adenocarcinoma : HCT 116
Normal human Keratinocytes : HaCaT
Normal human Fibroblasts : BJ
Human white pre-adipocyte subcutaneous : HWP cryopreserved / HPrAD
Human Fibroblasts : NHDF
Human Keratinocytes : NHEK
Human induced pluripotent stem cells : hiPS
Human sebocytes from induced pluripotent stem cells : PCi-SEB
Complete List of Tested Cells [PDF]
* Proof of Concept:
・In Vivo-like Morphology of Adipocytes [PDF]
・Increased Expression of Differentiation Genes [PDF]
・Higher Lipogenesis Activity [PDF]
・Maturation of Functional Human Adipocytes [PDF]
・Application: Regulation of Lipid Accumulation [PDF]
Compatible with Most Downstream Applications
BIOMIMESYS® hydroscaffold has many properties (transparent, porous, biodegradable, & solid) making it ideal for use with numerous downstream applications as shown below.
- Hyaluronic acid (HA) (1.6MDa) grafted with RGDS
- Adipic acid dihydrazide (ADH) as crosslinker
- Collagen Type I & VI
- Young’s modulus: ~0.5kPa – different stiffness is available through customization
- Porosity: 120±50µm
BIOMIMESYS® Adipose Tissue
24 Hydrogel / 96 Well
- SKU: BIO_ADI_96_24_transp
- SKU: BIO_ADI_96_24_black
96 Hydrogel / 96 Well
- SKU: BIO_ADI_96_96_transp
- SKU: BIO_ADI_96_96_black
References & Literature
1. Louis, F. et al. A biomimetic hydrogel functionalized with adipose ECM components as a microenvironment for the 3D culture of human and murine adipocytes. Biotechnol Bioeng 114, 1813–1824 (2017) doi: 10.1002/bit.26306.
2. Neels, J. G., Thinnes, T. & Loskutoff, D. J. Angiogenesis in an in vivo model of adipose tissue development. FASEB J 18, 983–985 (2004) doi: 10.1096/fj.03-1101fje.
3. Cristancho, A. G. & Lazar, M. A. Forming functional fat: a growing understanding of adipocyte differentiation. Nat Rev Mol Cell Biol 12, 722–734 (2011) doi: 10.1038/nrm3198.
4. Dalby, M. J., Gadegaard, N. & Oreffo, R. O. C. Harnessing nanotopography and integrin-matrix interactions to influence stem cell fate. Nat Mater 13, 558–569 (2014) doi: 10.1038/nmat3980.
5. McBeath, R., Pirone, D. M., Nelson, C. M., Bhadriraju, K. & Chen, C. S. Cell shape, cytoskeletal tension, and RhoA regulate stem cell lineage commitment. Dev Cell 6, 483–495 (2004) doi: 10.1016/S1534-5807(04)00075-9.
6. Wang, L., Johnson, J. A., Zhang, Q. & Beahm, E. K. Combining decellularized human adipose tissue extracellular matrix and adipose-derived stem cells for adipose tissue engineering. Acta Biomater 9, 8921–8931 (2013) doi: 10.1016/j.actbio.2013.06.035.
FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES