ALyS™505N is a serum-free and xeno-free medium used for the expansion and activation of human lymphocytes. It is available in four different IL-2 concentrations, suitable for activating or expanding T cells from human lymphocytes. Higher concentrations of IL-2 activate T cells, while lower concentrations are utilized for expansion. Gas-permeable culture bags are available for IL-2 free and 175 uL/mL mediums. Furthermore, higher cell proliferation can be achieved using a small amount of Artificial Serum. ALyS™705 is a serum-free and animal-free medium, chemically defined, used for the expansion and activation of human lymphocytes. Unlike ALyS™505N, ALyS™705 achieves an animal-free culture environment by utilizing a high molecular polymer instead of albumin, which in turn reduces the shear stress generated between cells. When supplemented with Artificial Serum., ALyS™705 has shown higher proliferation rates than ALyS™505N.