HemEX™ - Type9A
Mouse HSC expansion basal medium
Product Basics
HemEX™ – Type9A is a basal medium specifically designed for the expansion of mouse hematopoietic stem cells (HSCs) isolated from bone marrow. Contaminants derived from albumin can trigger the differentiation of HSCs. By substituting albumin with polyvinyl alcohol (PVA), HemEX™ – Type9A can maintain HSCs in an undifferentiated state for over one month during expansion culture.
Key Features
- Serum-free formulation
- Can maintain HSCs in an undifferentiated state for over one month of expansion culture
- Verified proliferation of mouse hematopoietic stem (HSCs) and progenitor cells (HSPCs)
- FACS purification not necessary
- Developed under the supervision of Prof. Hiromitsu Nakauchi (Stanford University) and Prof. Satoshi Yamazaki (Tokyo University), based on the Wilkinson et al. 2019 publication
Technical Information
Maintaining the Undifferentiated State
HemEX™ – Type9A utilizes polyvinyl alcohol instead of albumin to minimize oxidation reactions and suppress the differentiation of HSCs.
Fig.1 Flow cytometry analysis of mouse HSCs that were cultured in HemEx®-Type9A supplemented with 10ug/mL of stem cell factor cytokines and 100ug/mL of thrombopoietin (TPO) for 7 days using U-bottom plates. Based on the characteristics of Lin – / Sca-1 + / c-Kit +, the cultured cells maintained their stemness.
Formulation
Based on the Wilkinson et al 2019 publication.
**Requires the addition of cytokines.
**Penicillin and streptomycin are not in HemEx™ but maybe add based on your needs.
Specification
- Size: 100mL (bottle)
- Storage temperature: 2-8°C
- Shelf life: 1 year following manufacture date
- Manufactured by: Cell Science and Technology Institute
Pricing
HemEX™ - Type9A
- Mouse HSC expansion basal medium
- SKU: A5P00P01C
- Size: 100ml
- Price:
$200.00→ $180.00
References
- .Wilkinson, A. C. et al. Long-term ex vivo haematopoietic-stem-cell expansion allows nonconditioned transplantation. Nature 571, 117–121 (2019) doi: 10.1038/s41586-019-1244-x.
- Wilkinson, A. C., Ishida, R., Nakauchi, H. & Yamazaki, S. Long-term ex vivo expansion of mouse hematopoietic stem cells. Nature Protocols 15, 628–648 (2020) doi: 10.1038/s41596-019-0263-2.
- Ochi, K., Morita, M., Wilkinson, A. C., Iwama, A. & Yamazaki, S. Non-conditioned bone marrow chimeric mouse generation using culture-based enrichment of hematopoietic stem and progenitor cells. Nature Communications 12, 3568 (2021) doi: 10.1038/s41467-021-23763-z.
- Kawano, H. et al. Mitochondrial Transfer to Host Cells from Ex Vivo Expanded Donor Hematopoietic Stem Cells. Cells 12, (2023) doi: 10.3390/cells12111473.
- Natalia Skinder, Irene Sanz Fernández, Albertien Dethmers-Ausema, Ellen Weersing, & Gerald de Haan. CD61 identifies a superior population of aged murine hematopoietic stem cells and is required to preserve quiescence and self-renewal. bioRxiv 2023.08.14.553203 (2023) doi: 10.1101/2023.08.14.553203.
- Wilkinson, A. C., Igarashi, K. J. & Nakauchi, H. Haematopoietic stem cell self-renewal in vivo and ex vivo. Nature Reviews Genetics 21, 541–554 (2020) doi: 10.1038/s41576-020-0241-0.
- Igarashi, K. J. et al. Physioxia improves the selectivity of hematopoietic stem cell expansion cultures. Blood Advances 7, 3366–3377 (2023) doi: 10.1182/bloodadvances.2023009668.
- Shiroshita, K. et al. A culture platform to study quiescent hematopoietic stem cells following genome editing. Cell Reports Methods 2, 100354 (2022) doi: 10.1016/j.crmeth.2022.100354.
- Svendsen Flohr, Arthur. A Matter of Timing: A Genome-Wide Investigation of Hematopoietic Stem Cell Aging. (University of Groningen, 2022) doi: 10.33612/diss.223298967.
- Ochi, Kiyosumi, Morita, Maiko, Wilkinson, Adam, Iwama, Atsushi, & Yamazaki, Satoshi. Cell culture-based enrichment of mouse hematopoietic stem and progenitor cells,. Protocol Exchange (2021) doi: 10.21203/rs.3.pex-1481/v1.
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FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES