It is a long established fact that a reader of a page when looking at its layout.

    HemEX™ - Type9A

    Mouse HSC expansion basal medium

    Upgrade version of HemEX™-Type9A now available.  Click Here for press release.

    Product Basics

    HemEX™ – Type9A is a basal medium specifically designed for the expansion of mouse hematopoietic stem cells (HSCs) isolated from bone marrow. Contaminants derived from albumin can trigger the differentiation of HSCs. By substituting albumin with polyvinyl alcohol (PVA), HemEX™ – Type9A can maintain HSCs in an undifferentiated state for over one month during expansion culture.

    Key Features

    Technical Information

    Maintaining the Undifferentiated State

    HemEX™ – Type9A utilizes polyvinyl alcohol instead of albumin to minimize oxidation reactions and suppress the differentiation of HSCs.


    Fig.1 Flow cytometry analysis of mouse HSCs that were cultured in HemEx®-Type9A supplemented with 10ug/mL of stem cell factor cytokines and 100ug/mL of thrombopoietin (TPO) for 7 days using U-bottom plates. Based on the characteristics of Lin – / Sca-1 + / c-Kit +, the cultured cells maintained their stemness.


    Based on the Wilkinson et al 2019 publication.
    **Requires the addition of cytokines.
    **Penicillin and streptomycin are not in HemEx™ but maybe add based on your needs.


    • Size: 100mL (bottle)
    • Storage temperature: 2-8°C
    • Shelf life: 1 year following manufacture date
    • Manufactured by: Cell Science and Technology Institute


    Upgrade version of HemEX™-Type9A now available.  Click Here for press release.

    HemEX™ - Type9A

    • SKU: A5P00P01C
    • Size: 100ml
    • Price: $200.00$180.00


    1. .Wilkinson, A. C. et al. Long-term ex vivo haematopoietic-stem-cell expansion allows nonconditioned transplantation. Nature 571, 117–121 (2019) doi: 10.1038/s41586-019-1244-x.
    2. Wilkinson, A. C., Ishida, R., Nakauchi, H. & Yamazaki, S. Long-term ex vivo expansion of mouse hematopoietic stem cells. Nature Protocols 15, 628–648 (2020) doi: 10.1038/s41596-019-0263-2.
    3. Ochi, K., Morita, M., Wilkinson, A. C., Iwama, A. & Yamazaki, S. Non-conditioned bone marrow chimeric mouse generation using culture-based enrichment of hematopoietic stem and progenitor cells. Nature Communications 12, 3568 (2021) doi: 10.1038/s41467-021-23763-z.
    4. Kawano, H. et al. Mitochondrial Transfer to Host Cells from Ex Vivo Expanded Donor Hematopoietic Stem Cells. Cells 12, (2023) doi: 10.3390/cells12111473.
    5. Skinder, N., Fernandez, I. S., Dethmers-Ausema, A., Weersing, E. & de Haan, G. CD61 identifies a superior population of aged murine HSCs and is required to preserve quiescence and self-renewal. Blood advances 8, 99–111 (2024) doi: 10.1182/bloodadvances.2023011585.
    6. Wilkinson, A. C., Igarashi, K. J. & Nakauchi, H. Haematopoietic stem cell self-renewal in vivo and ex vivo. Nature Reviews Genetics 21, 541–554 (2020) doi: 10.1038/s41576-020-0241-0.
    7. Igarashi, K. J. et al. Physioxia improves the selectivity of hematopoietic stem cell expansion cultures. Blood Advances 7, 3366–3377 (2023) doi: 10.1182/bloodadvances.2023009668.
    8. Shiroshita, K. et al. A culture platform to study quiescent hematopoietic stem cells following genome editing. Cell Reports Methods 2, 100354 (2022) doi: 10.1016/j.crmeth.2022.100354.
    9. Svendsen Flohr, Arthur. A Matter of Timing: A Genome-Wide Investigation of Hematopoietic Stem Cell Aging. (University of Groningen, 2022) doi: 10.33612/diss.223298967.
    10. Ochi, Kiyosumi, Morita, Maiko, Wilkinson, Adam, Iwama, Atsushi, & Yamazaki, Satoshi. Cell culture-based enrichment of mouse hematopoietic stem and progenitor cells,. Protocol Exchange (2021) doi: 10.21203/rs.3.pex-1481/v1.