iMatrix-411 is a recombinant Laminin-411 E8 fragment protein used as a cell culture matrix. Laminin-411 is a predominant isoform found in the basal membrane of the endothelial cells in capillary vessels, and it mainly binds to the cell surface transmembrane receptors integrin α6β1 and α71β1.¹ The E8 fragment maintains full binding activity toward integrin, yet lacks binding activities to other components. The process of switching defined matrices is a critical tool for steering cells towards their intended fate.² Studies have demonstrated that switching the extracellular matrix (ECM) from laminin-511 E8 fragment (iMatrix-511) to laminin-411 E8 during differentiation efficiently induces endothelial progenitor cells from iPS cells.
¹ Nishiuchi, R. et al. Matrix Biol. 25, 189-197-,2006
² Ohta R et al., Scientific Reports, 6(35680), 1-12, 2016
Phenotypes of endothelial cells induced from pluripotent stem cells on iMatrix-411 coated plate
References & Literature
Takayama K. et al., Laminin 411 and 511 promote the cholangiocyte differentiation of human induced pluripotent stem cells. Biochemical and biophysical research communications, 474 (1), 91-96, 2016
Ohta R et al., Laminin-guided highly efficient endothelial commitment from human pluripotent stem cells. Scientific Reports, 6 (35680), 1-12, 2016
FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES