iMatrix 411
Induction of vascular endothelial cells from human PSCs
Product Basics
iMatrix-411 is a recombinant Laminin-411 E8 fragment protein used as a cell culture matrix. Laminin-411 is a major isoform that lines the basal membrane of the endothelial cells in capillary vessels and binds mainly to the cell surface transmembrane receptors integrin α6β1 and α71β1.¹ The E8 fragment retains full binding activity toward integrin but lacks binding activities to other components. The switching of defined matrices is an important tool for guiding cells towards their desired fate.² Studies have shown that changing the ECM from laminin-511 E8 fragment (iMatrix-511) to laminin-411 E8 during differentiation efficiently induces endothelial progenitor cells from iPS cells.
¹ Nishiuchi, R. et al. Matrix Biol. 25, 189-197-,2006
² Ohta R et al., Scientific Reports, 6(35680), 1-12, 2016
Key Features
- Switching from laminin-511 E8 to laminin-411 E8 during differentiation induces endothelial progenitor cells
- Highly purified and refined human laminin-411 E8 fragment expressed by CHO-S cells
- Feeder-free cell culture
Specification
- Format: Liquid Solution (175 ug/vial @0.5 mg/mL), Solvent: PBS (-)
- Storage temperature: 2-15 °C
- Manufactured by: Nippi, Inc. 1-1-1 Senju-Midoricho, Adachi-ku, Tokyo, Japan
Pricing
iMatrix-411
- Expressed by CHO-S Cell
- Liquid Solution (175 ug/vial @0.5 mg/mL), Solvent: PBS (-)
- 1mg of iMatrix-411 is enough to coat more than 35. 6-well plates for normal use
- Recommended concentration: 0.5µg/cm²
- SKU: N-892043
- Price: $80.00
- Incl. US (Continental) shipping fee
- * Only one purchase per lab *
350ug (175ug x 2 tubes)
- SKU: N-892041
- Price: $495.00
1050ug (175ug x 2 tubes)
- SKU: N-892042
- Price: $1,320.00
References & Literature
Takayama K. et al., Laminin 411 and 511 promote the cholangiocyte differentiation of human induced pluripotent stem cells. Biochemical and biophysical research communications, 474 (1), 91-96, 2016
Ohta R et al., Laminin-guided highly efficient endothelial commitment from human pluripotent stem cells. Scientific Reports, 6 (35680), 1-12, 2016
Other Documents
Related Products
FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES