CELLBANKER® 2 is a ready-to-use, serum-free cell cryopreservation medium formulated for a broad spectrum of mammalian cell cultures. The unique formulation enables stable cryopreservation and high viability after the freeze-thaw procedure, without the potential risk of serum contamination.
|Cells||Preservation period (Year)||Viability of cells (%)|
|Preserved at -80℃|
|EBV transformed cell||5||90|
For optimum results, cells for cryopreservation should be in log phase of growth. Similar or other standard freezing protocols may be substituted.
- Examine and make sure the cell culture is free of contamination, in healthy and at proper confluency.
- Perform a cell count to determine the viability of cells.
- Centrifuge at 1,000 – 2,000 rpm, 4°C for 3 to 5 minutes to gently pellet the cells. Remove the supernatant with an aspirator.
- Gently suspend STEM-CELLBANKER® GMP grade cryopreservation medium (1 mL for 5×10⁵ – 5×10⁶ cells).
- Transfer 1 mL of the cell suspension to cryopreservation vial labeled with appropriate information (the cell line name, concentration, passage date etc.).
- Place the vials directly in -80℃ for storage.
- (OPTIONAL) Transfer the frozen vials to a liquid nitrogen storage tank after the vials have been frozen for at least 24 hours.
IMPORTANT: Optimum protocol may change with the cell types.
- Remove the cryopreservation vial from the freezer and quickly thaw cells in a 37°C shaking water bath or shake by hand.
- Transfer the content to a centrifugation tube then immediately dilute and gently mix with 10mL of complete cell culture medium. Using CELLOTION® instead of complete culture medium will prevent adhesion of cells to the wall of the tube, increasing the recovery rate.
- Centrifuge cells at 1,000 – 2,000 rpm, 4°C for 3 to 5 minutes. Remove the supernatant with an aspirator.
- Gently resuspend the cells with an appropriate volume of complete cell culture medium then plate in a culture flask or plate
- Continue the culture procedures according to standard protocols.
- Manufactured By : Zenogen Pharma Co., Ltd
- Size : 20mL & 100mL
- Storage and Stability : 2 to 8℃ or below -20℃
- Expiration date : 3 years after the date of manufacture
- Disclaimer: For research purposes only
References & Literature
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Yoshimatsu, S. et al. Establishing an induced pluripotent stem cell line from neonatal common marmoset fibroblasts by an all-in-one episomal vector approach. Stem Cell Research 53, 102380 (2021) doi: 10.1016/j.scr.2021.102380.
Hu, Y. et al. Lung cancer organoids analyzed on microwell arrays predict drug responses of patients within a week. Nature Communications 12, 2581 (2021) doi: 10.1038/s41467-021-22676-1.
Wei Yanxing et al. Efficient derivation of human trophoblast stem cells from primed pluripotent stem cells. Science Advances 7, eabf4416 doi: 10.1126/sciadv.abf4416.
Ma, L. et al. CD146 controls the quality of clinical grade mesenchymal stem cells from human dental pulp. Stem Cell Research & Therapy 12, 488 (2021) doi: 10.1186/s13287-021-02559-4.
Bajaj, S. et al. Neurotransmitter signaling regulates distinct phases of multimodal human interneuron migration. The EMBO Journal 40, e108714 (2021) doi: 10.15252/embj.2021108714.
Deng, B. et al. Peripheral leukemia burden at time of apheresis negatively affects the clinical efficacy of CART19 in refractory or relapsed B-ALL. Molecular Therapy – Methods & Clinical Development 23, 633–643 (2021) doi: 10.1016/j.omtm.2021.10.006.
FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES