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    ALyS™505NK Series

    Serum-free cell culture medium for activation and expansion of Human NK cells

    Product Basics

    ALyS505NK-AC and ALyS505NK-EX are serum-free, xeno-free cell culture media optimized for human natural killer (NK) cells derived from peripheral blood mononuclear cells (PBMCs). NK cells are crucial for innate immunity. Unlike T cells, they can damage virus-infected cells and cancer cells without prior sensitization.
    Many applications necessitate the expansion of NK cells. However, in vitro cultivation of NK cells often results in a low expansion rate, an exhausted phenotype due to long-term expansion, and overgrowth of T cells if present in the initial culture. By employing ALyS505NK-AC for activation and ALyS505NK-EX for expansion, one can attain a highly pure, NK-rich population of cells in 14 days without the need for sorting with beads or flow cytometry.
    Artificial Serum is recommended for the primary culture derived from PBMC. Both ALyS505NK-AC and ALyS505NK-EX are available with or without IL-2 (1000IU/mL).

    Key Features

    Technical Information

    Performance data of Alys505NK-AC&EX

    Robust Proliferation

    As shown in Fig. 1, the proliferation of PBMC cultured in ALyS505NK-AC & EX over 14 days was higher than that in competitor G’s medium.

    Highly efficient proliferation ratio of NK cells

    In Fig. 2 and 3, PBMCs were collected from three donors and cultured with ALyS505NK-AC & EX and competitor G’s medium. As depicted in Fig. 2, the number of NK cells obtained on day 14 was significantly higher when cultured with ALyS505NK-AC & EX. Furthermore, as shown in Fig. 3, the ratio of NK cells to T cells was between 55-75% and 45-25% with ALyS505NK-AC & EX. In contrast, only 25-45% of NK cells were obtained after culturing in competitor G’s medium. Despite some noticeable variation among donors, ALyS505NK-AC & EX demonstrated highly efficient proliferation of NK cells and a high NK cell ratio.

    Phenotype of NK cells are maintained

    In Fig. 4, the cytotoxicity level of NK cells was evaluated by incubating them with K562 for four hours at varying E/T ratios. The NK cells cultured from ALyS505NK-EX exhibited cytotoxic activity even at low E/T ratios.


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    01600P02ALyS505NK-ACIL-2 free (Activation)200mlBottle4°C
    01610P02ALyS505NK-AC1000IL-2 concentration 1,000lU/mL (Activation)200mlBottle4°C
    01400P10/01400C10ALyS505NK-EXIL-2 free (Expansion)1LBottle/Culture Bag4°C
    01410P10/01410C10ALyS505NK-EX1000IL-2 concentration 1,000lU/mL (Expansion)1LBottle/Culture Bag4°C


    • Manufactured by: Cell Science and Technology Institute


    ALyS™505NK Series


    IL-2 Free
    • SKU: 01600P02
    • Size: 200ml (Bottle)
    • Price: $100.00$90.00


    IL-2 concentration 1,000lU/mL
    • SKU: 01610P02
    • Size: 200ml (Bottle)
    • Price: $135.00$121.00


    IL-2 Free
    • SKU: 01400P10
    • Size: 1L (Bottle)
    • Price:$150.00$135.00
    • SKU: 01400C10
    • Size: 1L (Culture Bag)
    • Price: Contact us


    IL-2 concentration 1,000lU/mL
    • SKU: 01410P10
    • Size: 1L (Bottle)
    • Price: $255.00$230.00
    • SKU:01410C10
    • Size: 1L (Culture Bag)
    • Price: Contact us


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    2. Singh TD, Lee J, Jeon YH. Noninvasive Imaging of Natural Killer Cell-Mediated
      Apoptosis in a Mouse Tumor Model. Methods Mol Biol. 2016;1441:297-306.
    3. Niu, C. et al. Low-dose bortezomib increases the expression of NKG2D and DNAM-1 ligands and enhances induced NK and γδ T cell-mediated lysis in multiple myeloma. Oncotarget 8, 5954–5964 (2017) doi: 10.18632/oncotarget.13979.
    4. Yu, H. et al. Large scale ex vivo expansion of clinical‑grade effector cells for adoptive immunotherapy. Exp Ther Med 14, 5678–5686 (2017) doi: 10.3892/etm.2017.5228.
    5. Jung, I. H. et al. In Vivo Study of Natural Killer (NK) Cell Cytotoxicity Against Cholangiocarcinoma in a Nude Mouse Model. In Vivo 32, 771 (2018) doi: 10.21873/invivo.11307.
    6. Lai, H. et al. Selenium-containing ruthenium complex synergizes with natural killer cells to enhance immunotherapy against prostate cancer via activating TRAIL/FasL signaling. Biomaterials 219, 119377 (2019) doi: 10.1016/j.biomaterials.2019.119377.
    7. Nakamura, Y. et al. Natural killer cells impede the engraftment of cardiomyocytes derived from induced pluripotent stem cells in syngeneic mouse model. Scientific Reports 9, 10840 (2019) doi: 10.1038/s41598-019-47134-3.
    8. Liu, X., Sun, T., Ge, Q. & Zhu, J. Construction of Novel BispecificSingle-Domain Antibodies (BiSdAbs) with Potent Antiangiogenic Activities. Pharmaceutical Fronts 2, e64–e76 (2020) doi: 10.1055/s-0040-1708527.
    9. Chang, J. et al. Diffracted X-ray blinking measurements of interleukin 15 receptors in the inner/outer membrane of living NK cells. Biochemical and Biophysical Research Communications 556, 53–58 (2021) doi: 10.1016/j.bbrc.2021.03.144.
    10. Jung, D. et al. Ex vivo expanded allogeneic natural killer cells have potent cytolytic activity against cancer cells through different receptor-ligand interactions. Journal of Experimental & Clinical Cancer Research 40, 333 (2021) doi: 10.1186/s13046-021-02089-0.
    11. Mikawa, S., Matsuda, A., Kamemori, Y., Asanuma, S. & Kitagawa, H. Enhancement of natural killer cell activity by oral administration of a fermented soybean product in dogs. Open Veterinary Journal 11, 394–400 (2021) doi: 10.5455/OVJ.2021.v11.i3.10.

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