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    Serum free medium for Human Fibroblast

    Product Basics

    HFDM®-1 is a chemically defined medium for culturing human normal dermal fibroblast. (HNDF). Fibroblast cultured with this medium under serum free condition can proliferate as well as DMEM supplemented with 10% serum. For expansion of fibroblast cells less serum (0.5 – 2.0%) is required compared to conventional methods.

    Key Features

    * * CSTI Artificial Serum can be used instead of serum and obtain similar results while maintaining a serum free cell culture system **

    Technical Information

    Human fibroblast cell growth with HFDM-1

    Fig. 1
    Human normal dermal fibroblasts cultured in HFDM-1(+) under serum free can proliferate as well as DMEM supplemented with 10% serum.

    Fig. 2
    Human normal dermal fibroblasts cultured in HFDM-1(+) with various concentration of human serum. Supplementing HFDM™-1 with a range of 0.5 to 2% human serum showed great expansion.

    Cells Tested

    Normal human dermal fibroblasts (NHDFs)
    Primary human dermal fibroblasts
    Human fibroblast from oral tissue. Human gingival fibroblasts
    Cardiac fibroblasts (CFs autologous primary fibroblasts)
    Cancer-associated fibroblasts (CAF)
    Plumonary fibroblast from lung cancer patients
    human intestinal organoid-derived fibroblasts
    U251 human glioma cells.

    Mouse PBMC and fibroblast from tail
    mice fibroblast sheet
    mouse embryo fibroblasts (MEF) clones
    Rat multilayered fibroblast sheets derived from autologous oral mucosal
    C6 rat glioma cells


    • Size: 500mL/1000mL
    • Storage temperature: 2-8°C
    • Shelf life: 12 months following manufacture date
    • Manufactured by: Cell Science and Technology Institute




    • SKU: 2102P05
    • Price: $105.00$95.00


    • SKU: 2102P10
    • Price: $133.00$120.00


    1. Mitui, M., Takahashi, K., Kobayashi, K. & Matsuoka, T. Human lung fibroblasts cultivated with HFDM-1 reduced both the secreted PAI-1 and the surface UPA activities. Tiss Cult Res 26, 133–142 (2007) doi:10.11418/jtca.26.133
    2. Hirobe, T., Hasegawa, K., Furuya, R., Fujiwara, R. & Sato, K. Effects of fibroblast-derived factors on the proliferation and differentiation of human melanocytes in culture. Journal of Dermatological Science 71, 45–57 (2013) doi: 10.1016/j.jdermsci.2013.03.012.
    3. Morimoto, N. et al. An exploratory clinical study on the safety and efficacy of an autologous fibroblast-seeded artificial skin cultured with animal product-free medium in patients with diabetic foot ulcers. International Wound Journal 11, 183–189 (2014) doi: 10.1111/j.1742-481X.2012.01064.x.
    4. Ejiri, H. et al. Use of synthetic serum-free medium for culture of human dermal fibroblasts to establish an experimental system similar to living dermis. Cytotechnology 67, 507–514 (2015) doi: 10.1007/s10616-014-9709-0.
    5. Mizoguchi, T. et al. Treatment of Cutaneous Ulcers with Multilayered Mixed Sheets of Autologous Fibroblasts and Peripheral Blood Mononuclear Cells. Cellular Physiology and Biochemistry 47, 201–211 (2018) doi: 10.1159/000489767.
    6. Mizoguchi, T. et al. Autologous fibroblasts, peripheral blood mononuclear cells, and fibrin glue accelerate healing of refractory cutaneous ulcers in diabetic mice. Am J Transl Res 10, 2920–2928 (2018). PMCID: PMC6176242
    7. Nanki, K. et al. Divergent Routes toward Wnt and R-spondin Niche Independency during Human Gastric Carcinogenesis. Cell 174, 856-869.e17 (2018) doi: 10.1016/j.cell.2018.07.027.

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