CiMS™ is a serum-free cell culture medium for human mesenchymal stem cells (hMSC). The CiMS™-BM (base medium) is optimized for multiple passage expansions of various undifferentiated hMSCs. Pre-coating of culture vessels with an attachment matrix is not required, thereby improving efficiency. Furthermore, by adding either an animal-free supplement (CiMS™-sAF) or a xeno-free supplement (CiMS™-sXF), which are optimized for CiMS™-BM, long-term serum-free culture with high proliferation can be achieved.
Proliferation activity of AT-MSCs was evaluated by population doubling level (PDL). Cells cultured in CiMS™-BM supplemented with either CiMS™-sXF or CiMS™-sAF without matrices have shown high proliferation through passages. Results are similar to cells cultured in MSC-T4 with fibronectin coated plates.
Osteogenic, adipogenic and chondrogenic differentiation capability of AT-MSCs cultured in CiMS™-BM with CiMS™-sAF supplement were confirmed by Alizarin Red S staining, Oil Red O staining, and toluidine blue staining, respectively. The same differentiation capability was confirmed with CiMS™-sXF has been confirmed.
|Cat#||Product||Description||Size||Contents||Storage Upon Arrival|
|A2G00P05C||CiMS™-BM||Basal medium||500ml||1 bottle||4°C, protect from light|
|A2G10P1CC||CiMS™-sXF||Xeno-free supplement||10ml||1 bottle||Below -20°C|
|A2G20P1CC||CiMS™-sAF||Animal-free supplement||10ml||1 bottle||Below -20°C|
- Manufactured by: Cell Science and Technology Institute
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FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES