CiMS™ and CiMS™-U
Animal-free culture medium for human MSC. The formulation of CiMS™-U is optimized for hMSC derived from umbilical cord tissue.
Product Basics
CiMS™ is a serum-free cell culture medium for human mesenchymal stem cells (hMSC). The CiMS™-BM (base medium) is optimized for multiple passage expansions of various undifferentiated hMSCs. Pre-coating of culture vessels with an attachment matrix is not required, thereby improving efficiency. With the animal-free supplement (CiMS™-sAF), which is optimized for CiMS™-BM, long-term serum-free culture with high proliferation can be achieved.
CiMS™-U is a sister version of CiMS™, specially formulated for optimum culture of umbilical cord tissue derived hMSC (UC-hMSC). CiMS™-U can be used for hMSCs from other tissues, such as adipose and bone-marrow.
Key Features
- Animal-free culture medium designed for hMSC
- Facilitates the expansion of various hMSCs in an undifferentiated state
- Eliminates the need for pre-coating culture vessels, thereby improving efficiency
- CiMS™-U offers optimized growth of umbilical tissue derived hMSC
- Enables long-term culture with high proliferation of various hMSCs
- Allows differentiation into several desired lineages
- Manufactured in compliance with Japanese GMP guidelines (CiMS™)
Technical Information
Fig. 1
hMSCs cultured in CiMS™ (day 4) or CiMS™-U (day 3) are shown. Both maintained normal morphology and adherence to the culture dish.
Fig. 2
Proliferation activity of AT-MSCs was evaluated by population doubling level (PDL). Cells cultured in CiMS™-BM supplemented with CiMS™-sAF without matrices have shown high proliferation through passages. Results are similar to cells cultured in MSC-T4 medium with fibronectin coated plates. UC-MSCs cultured in CiMS™- U proliferated faster than in MEMα medium supplemented with 10% FBS.
Fig. 3
Osteogenic, adipogenic and chondrogenic differentiation capability of AT-MSCs cultured in CiMS™-BM with CiMS™-sAF supplement were confirmed by Alizarin Red S staining, Oil Red S staining, and toluidine blue staining, respectively. UC-MSCs cultured with CiMS™- U showed the same multiple lineage capability.
Fig. 4
The MSC marker profile was maintained in UC-MSCs after 12 days of culture in CiMS™-U.
Specification
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| Cat# | Product | Description | Size | Contents | Storage Upon Arrival |
|---|---|---|---|---|---|
| A2G00P05C | CiMS™-BM | Basal medium | 500mL | 1 bottle | 4°C, protect from light |
| A2G20P1CC | CiMS™-sAF | Animal-free supplement | 10mL | 1 bottle | Below -20°C |
| A2J10P05C | CiMS™-U/BM | Basal medium | 500mL | 1 bottle | 4°C, protect from light |
| A2J20P1CC | CiMS™-U/sAF | Animal-free supplement | 10mL | 1 bottle | Below -20°C |
- Manufactured by: Cell Science and Technology Institute
Pricing
CiMS™ and CiMS™-U
- Serum-free culture medium for human MSC
- Comprised of the basal medium and supplement
CiMS™-BM
Base Medium
- SKU: A2G00P05C
- Size: 500mL
- Price:
$216.00→ $180.00
CiMS™-sAF
Animal Free Supplement
- SKU: A2G20P1CC
- Size: 10mL
- Price:
$836.00→ $695.00
CiMS™-U/BM
Base Medium
- SKU: A2J10P05C
- Size: 500mL
- Price: $195.00
CiMS™-U/sAF
Animal Free Supplement
- SKU: A2J20P1CC
- Size: 10mL
- Price: $1260.00
References
- Sato, K. et al. Serum-free isolation and culture system to enhance the proliferation and bone regeneration of adipose tissue-derived mesenchymal stem cells. In Vitro Cellular & Developmental Biology – Animal 51, 515–529 (2015) doi: 10.1007/s11626-014-9860-0.
- Murabayashi, D., Mochizuki, M., Tamaki, Y. & Nakahara, T. Practical methods for handling human periodontal ligament stem cells in serum-free and serum-containing culture conditions under hypoxia: implications for regenerative medicine. Hum Cell 30, 169–180 (2017) doi: 10.1007/s13577-017-0161-2.
- Piao, L., Huang, Z., Inoue, A., Kuzuya, M. & Cheng, X. W. Human umbilical cord-derived mesenchymal stromal cells ameliorate aging-associated skeletal muscle atrophy and dysfunction by modulating apoptosis and mitochondrial damage in SAMP10 mice. Stem Cell Research & Therapy 13, 226 (2022) doi: 10.1186/s13287-022-02895-z.
- Yamamoto, T. et al. A Novel Approach for Determining the Critical Quality Attributes of Mesenchymal Stem Cells by Specifying Cell Population With Replication Potential. Stem Cells Translational Medicine 12, 169–182 (2023) doi: 10.1093/stcltm/szad005.
- Huang, Z. et al. Human umbilical cord-derived mesenchymal stromal cell exosomes ameliorate aging-associated skeletal muscle atrophy and dysfunction in SAMP10 mice. Stem Cell Research & Therapy 16, 410 (2025) doi: 10.1186/s13287-025-04477-1.
- Kawasumi, R. et al. Systemic administration of induced pluripotent stem cell-derived mesenchymal stem cells improves cardiac function through extracellular vesicle-mediated tissue repair in a rat model of ischemic cardiomyopathy. Regenerative Therapy 28, 253–261 (2025) doi: 10.1016/j.reth.2024.12.008.
- Ueno, K. et al. miR-765 induces angiogenesis by inhibiting dipeptidyl peptidase 4 and increasing fibroblast growth factor 2. BBA Advances 9, 100193 (2026) doi: 10.1016/j.bbadva.2026.100193.
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FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES