We are excited to introduce the HemEx™-TypeH, a specialized, serum-free medium developed for the expansion of human hematopoietic stem cells. Based on Iscove’s MDM formulation, this medium is designed to maintain long-term HSCs from both umbilical cord blood and peripheral blood stem cells.

Key Features of the HemEx™-TypeH:

• Serum-free and albumin-free for a more controlled culture environment
• Chemically defined to provide precise control over cell culture conditions
• Proven to support the proliferation of human HSCs and hematopoietic stem and progenitor cells

HemEx™-TypeH creates the optimal conditions for maintaining undifferentiated HSCs, offering flexibility to incorporate your choice of cytokines and small molecules. By replacing albumin with PCL-PVAc-PEG (Soluplus), the medium prevents HSC differentiation, even during extended culture periods.

References:

1. Sakurai, M. et al. Chemically defined cytokine-free expansion of human haematopoietic stem cells. Nature 615, 127–133 (2023) doi: 10.1038/s41586-023-05739-9.
2. Sakurai, M., Ishitsuka, K. & Yamazaki, S. Chemically defined cytokine-free expansion of human haematopoietic stem cells. Protocol Exchange (2023) doi: 10.21203/rs.3.pex-2163/v1.
3. Ishitsuka, K., Nishikii, H., Kimura, T., Sugiyama-Finnis, A. & Yamazaki, S. Purging myeloma cell contaminants and simultaneous expansion of peripheral blood-mobilized stem cells. Experimental Hematology 131, 104138 (2024) doi: 10.1016/j.exphem.2023.104138.

If you need further details on the HemEx™-TypeH, please do not hesitate to reach out to us at: orders@iwai-chem.com or use below contact form