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    CELLBANKER® 1

    Preserving standard animal cells and cell lines

    Product Basics

    CELLBANKER® 1 is a ready-to-use cell cryopreservation medium with serum containing formulation for broad spectrum of mammalian cell cultures. The unique formulation allows for stable cryopreservation and high viability after freeze-thaw procedure, even for sensitive cell lines.

    Key Features

    Technical Information

    Experimental results

    Cells Preservation period (Year) Viability of cells (%)
    Preserved at -80℃ Preserved at -196℃
    MOUSE
    Hybridoma 10 95 95
    Myeloma 10 90 90
    L929 10 90 90
    FM3A 5 90 90
    BALB/3T3 5 90 90
    M1 5 90 90
    YAC-1 5 90 -
    RAT
    RLC-16 5 90 90
    NRK 5 90 90
    PC-12 5 90 -
    HAMSTER
    CHO 5 90 90
    V79 5 90 90
    MONKEY
    COS-1 5 90 90
    Vero 5 90 90
    HUMAN
    Kidney-derived tumor cell 5 90 90
    EBV transformed cell 5 90 90
    HEL-derived fibroblast 5 90 90
    Melanoma 5 90 90
    Caco-2 3 90 -
    C-5 5 90 90
    CEM 5 90 90
    K562 10 90 90
    Jurkat 10 90 90
    BALL-1 5 90 90
    HUC-Fm 5 80 80

    List of Cells Tested [PDF] – Cell types are constantly being added. Please contact us if you cannot find your cell listed.

    Protocol

    Freezing

    For optimum results, cells for cryopreservation should be in log phase of growth. Similar or other standard freezing protocols may be substituted.

    1. Examine and make sure the cell culture is free of contamination, in healthy and at proper confluency.
    2. Perform a cell count to determine the viability of cells.
    3. Centrifuge at 1,000 – 2,000 rpm, 4°C for 3 to 5 minutes to gently pellet the cells. Remove the supernatant with an aspirator.
    4. Gently suspend STEM-CELLBANKER® GMP grade cryopreservation medium (1 mL for 5×10⁵ – 5×10⁶ cells).
    5. Transfer 1 mL of the cell suspension to cryopreservation vial labeled with appropriate information (the cell line name, concentration, passage date etc.).
    6. Place the vials directly in -80℃ for storage.
    7. (OPTIONAL) Transfer the frozen vials to a liquid nitrogen storage tank after the vials have been frozen for at least 24 hours.

    IMPORTANT: Optimum protocol may change with the cell types.

    Thawing

    1. Remove the cryopreservation vial from the freezer and quickly thaw cells in a 37°C shaking water bath or shake by hand.
    2. Transfer the content to a centrifugation tube then immediately dilute and gently mix with 10mL of complete cell culture medium. Using CELLOTION® instead of complete culture medium will prevent adhesion of cells to the wall of the tube, increasing the recovery rate.
    3. Centrifuge cells at 1,000 – 2,000 rpm, 4°C for 3 to 5 minutes. Remove the supernatant with an aspirator.
    4. Gently resuspend the cells with an appropriate volume of complete cell culture medium then plate in a culture flask or plate
    5. Continue the culture procedures according to standard protocols.

    Specification

    • Manufactured By : Zenogen Pharma Co., Ltd
    • Size : 20mL & 100mL
    • Storage and Stability : 2 to 8℃ or below -20℃
    • Expiration date : 3 years after the date of manufacture
    • Disclaimer: For research purposes only

    Pricing

    CELLBANKER® 1

    20ml size

    Price: $35.00

    100ml size

    Price: $150.00

    20ml size x 4

    Price: $135.00

    References & Literature

    Hwang, Y. S. et al. Reconstitution of prospermatogonial specification in vitro from human induced pluripotent stem cells. Nature Communications 11, 5656 (2020) doi: 10.1038/s41467-020-19350-3.

    Lee, H.-O. et al. Lineage-dependent gene expression programs influence the immune landscape of colorectal cancer. Nature Genetics 52, 594–603 (2020) doi: 10.1038/s41588-020-0636-z.

    Wang, Z. et al. mRNA vaccine-elicited antibodies to SARS-CoV-2 and circulating variants. Nature 592, 616–622 (2021) doi: 10.1038/s41586-021-03324-6.

    Shinozawa, T. et al. High-Fidelity Drug-Induced Liver Injury Screen Using Human Pluripotent Stem Cell–Derived Organoids. Gastroenterology 160, 831-846.e10 (2021) doi: 10.1053/j.gastro.2020.10.002.

    Parajuli, B. et al. Transnasal transplantation of human induced pluripotent stem cell-derived microglia to the brain of immunocompetent mice. Glia 69, 2332–2348 (2021) doi: 10.1002/glia.23985.

    Oguri, S. et al. Effect of varying storage conditions on diagnostic test outcomes of SARS-CoV-2. J Infect 83, 119–145 (2021) doi: 10.1016/j.jinf.2021.03.026.

    Yoshida, J. et al. Mitochondrial complex I inhibitors suppress tumor growth through concomitant acidification of the intra- and extracellular environment. iScience 24, 103497 (2021) doi: 10.1016/j.isci.2021.103497.

    Other Documents

    FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES