MC-210 is a cost-effective solution for eliminating mycoplasma contamination. This agent demonstrates high removal effects against common contaminants such as mycoplasma orale.
Mycoplasma contamination can alter an endless number of parameters in unpredictable ways. Mycoplasma often cohabits with your cells instead of killing your cultures and it skews titers before producing noticeable effects. It can interfere with cell migration, signal transduction, lymphocyte activation, and cytokine expression.
KAC’s MC-210 Mycoplasma Removal Agent(MRA) contains quinoline as an antibiotic due to its mycoplasmacidal properties. Quinoline inhibits mycoplasma DNA gyrase or the topoisomerase IV enzyme and interferes with DNA replication. Recommended treatment dose is 0.5 μg/mL for 1 week. Developed by Dainippon Pharmaceuticals, it has proven to be an effective elimination treatment with low cell toxicity for over 20 years.
Instructions for Use
1. Add the agent at a final concentration of 0.5µg/mL to cultured cells contaminated by mycoplasma and wait for one week.
Example: With 10mL of culture media in a 25cm² culture flask, add 0.1mL of the agent.
2. When the culture media is changed, add the agent at a comparable concentration.
3. After one week, add media without the agent and verify that there is no mycoplasma growth in the cell culture.
This agent is for research purposes and should be used only as a mycoplasmacidal agent for cell culture. Please strictly follow the required concentration for use (0.5µg/mL). If mycoplasma is still observed after treatment, increase the concentration to 1µg/mL.
Toxicity of this agent to cultured cells is low, and cytotoxicity was not observed at the required concentration. However, for specific functions of cells, please verify that there are no abnormalities after mycoplasma removal.
- Pack Size: 5 mL
- Concentration: 50 µg/mL
- Storage method: Stable at room temperature (4°C – 37°C). Please protect from light and store in the dark.
- Manufactured by: KAC Co., Ltd.
References & Literature
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FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES