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    iMatrix 511 Series

    Maintenance and expansion of pluripotent stem cells (PSCs)

    Product Basics

    iMatrix-511 is an innovative cell culture matrix compatible with a wide variety of cell types, and exceptionally well suited for pluripotent stem cells. This product is comprised of recombinant Laminin-511 E8 protein fragments which permit ES/iPS cells to be maintained in xeno-free culture conditions, enable the passaging of single cells, and provide greater adhesion than full-length Laminin, Vitronectin or Matrigel (Miyazaki et al., Nature Commun. 3; 1236 (2012))

    Key Features

    Technical Information

    Greater Cell Adhesion & Proliferation

    In the study referenced above, the Laminin-511 E8 fragment and other cell culture substrates were compared for their strength in adhering to hES cells. The horizontal axis of the graph shows the concentration of cell culture substrate, and the vertical axis, the OD value (optical density at 570nm). This result shows that the Laminin-511 E8 fragment adheres to cells more strongly than its competitors.

    In the same study cited above, Human ES cells were passaged on Laminin-511 E8 fragment and other cell culture substrates. Upon singly dispersing human ES cell colonies at the time of passage, it was confirmed that single cells adhere rapidly to Laminin-511 E8 and proliferate immediately.

    Maintain and Culture Single ES/iPS Cells with Greater Propagation

    With the conventional method, ES/iPS cells die when dispersed into single cells, making it necessary to maintain a cell colony when passaging.

    Using the Laminin-511 E8 fragment as a cell culture matrix enables you to culture single ES/iPS cells, thereby reducing the time required to optimize culture methods and drastically enhancing efficiency of cell cultures.

    Culture more ES/iPS cells using Laminin-511 E8 fragment

    The graph above compares the numbers of ES/iPS cells cultured by the conventional method (colony) for 30 days with those cultured by Laminin-511 E8 fragment. The results confirm that the number of cells multiplied by at least 200 when Laminin-511 E8 fragment was used.

    No pre-coating required

    • Reduces preparation time.
    • Reduces the amount to use by about half compared to pre-coating method.

    Reduces the amount to use by about half compared to pre-coating method.

    The pre-mixed method reduces the amount of substrate needed for maximum adhesion of hPSCs compared to pre-coat method.

    Specification

    • Format: Liquid Solution (175 ug/vial @0.5 mg/mL), Solvent: PBS (-)
    • Storage temperature: 2-15 °C
    • Manufactured by : Nippi, Inc. 1-1-1 Senju-Midoricho, Adachi-ku, Tokyo, Japan

    Pricing

    iMatrix-511

    • SKU: N-892013
    • Price: $80.00
    • Incl. US shipping fee
    • * Only one purchase per lab *
    350ug size (175ug x 2 tubes)
    • SKU: N-892011
    • Price: $270.00$216.00 
    1050ug size (175ug x 6 tubes)
    • SKU: N-892012
    • Price: $690.00$552.00 

    iMatrix-511 Silk

    • SKU: N-892022
    • Price: $80.00
    • Incl. US shipping fee
    • * Only one purchase per lab *
    1050ug size (175ug x 6 tubes)
    • SKU: N-892021
    • Price: $340.00 

    Easy iMatrix-511

    Easy iMatrix-511 (CHO-S Cell)

    • SKU: N-892019
    • Price: $80.00
    • Incl. US shipping fee
    • * Only one purchase per lab *
    • SKU: N-892018
    • Price: $120.00 

    Easy iMatrix-511 Silk (Transgenic silkworm cocoon)

    • SKU: N-892025
    • Price: $80.00
    • Incl. US shipping fee
    • * Only one purchase per lab *
    • SKU: N-892024
    • Price: $95.00

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    FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES