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    iMatrix 511 Series

    Maintenance and expansion of pluripotent stem cells (PSCs) & mesenchymal stem cells (MSCs)

    Product Basics

    The iMatrix-511 is an innovative cell culture matrix that is compatible with a wide variety of cell types. It is exceptionally well-suited for pluripotent and mesenchymal stem cells. This product consists of recombinant Laminin-511 E8 protein fragments, which allow ES/iPS cells and MSCs to be maintained in xeno-free culture conditions. It also enables the passaging of single cells and provides greater adhesion than full-length Laminin, Vitronectin, or Matrigel (Miyazaki et al., Nature Commun. 3; 1236 (2012)).

    Key Features

    Laminin 511

    Technical Information

    Greater Cell Adhesion & Proliferation

    In the referenced study above, the adhesion strength of the Laminin-511 E8 fragment and other cell culture substrates to hES cells were compared. The horizontal axis of the graph represents the concentration of the cell culture substrate, while the vertical axis represents the OD value (optical density at 570nm). These results demonstrate that the Laminin-511 E8 fragment adheres to cells more strongly than its competitors.

    In the same study mentioned above, human ES cells were passaged on Laminin-511 E8 fragment and other cell culture substrates. When human ES cell colonies were dispersed individually at the time of passage, it was observed that single cells adhered rapidly to Laminin-511 E8 and proliferated immediately.

    Maintain and Culture Single ES/iPS Cells with Greater Propagation

    With conventional methods, ES/iPS cells perish when dispersed into single cells, necessitating the maintenance of a cell colony during passaging.

    Using the Laminin-511 E8 fragment as a cell culture matrix enables the culturing of single ES/iPS cells, which in turn reduces the time required to optimize culture methods and drastically enhances the efficiency of cell cultures.

    Culture more ES/iPS cells using Laminin-511 E8 fragment

    The graph above compares the numbers of ES/iPS cells cultured by the conventional method (colony) for 30 days with those cultured using the Laminin-511 E8 fragment. The results confirm that the cell count increased by at least 200 times when the Laminin-511 E8 fragment was employed.

    No pre-coating required

    • Reduces preparation time.
    • Reduces the amount to use by about half compared to pre-coating method.

    Reduces the amount to use by about half compared to pre-coating method.

    The pre-mixed method reduces the amount of substrate needed for maximum adhesion of hPSCs compared to pre-coat method.

    Specification

    • Format: Liquid Solution (175 ug/vial @0.5 mg/mL), Solvent: PBS (-)
    • Storage temperature: 2-15 °C
    • Manufactured by : Nippi, Inc. 1-1-1 Senju-Midoricho, Adachi-ku, Tokyo, Japan

    Pricing

    iMatrix-511

    • SKU: N-892013
    • Price: $80.00
    • Incl. US (Continental) shipping fee
    • * Only one purchase per lab *
    350ug size (175ug x 2 tubes)
    • SKU: N-892011
    • Price: $270.00$216.00 
    1050ug size (175ug x 6 tubes)
    • SKU: N-892012
    • Price: $690.00$570.00

    iMatrix-511 Silk

    • SKU: N-892022
    • Price: $80.00
    • Incl. US (Continental) shipping fee
    • * Only one purchase per lab *
    1050ug size (175ug x 6 tubes)
    • SKU: N-892021
    • Price: $390.00 → $330.00

    Easy iMatrix-511

    Easy iMatrix-511 (CHO-S Cell)

    • SKU: N-892019
    • Price: $80.00
    • Incl. US (Continental) shipping fee
    • * Only one purchase per lab *
    • SKU: N-892018
    • Price: $235.00 

    Easy iMatrix-511 Silk (Transgenic silkworm cocoon)

    • SKU: N-892025
    • Price: $80.00
    • Incl. US (Continental) shipping fee
    • * Only one purchase per lab *
    • SKU: N-892024
    • Price: $190.00

    References & Literature

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    FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES