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    STEM-CELLBANKER®
    DMSO free GMP grade

    Preserving cells for regenerative medicine and cell therapy researches

    Product Basics

    STEM-CELLBANKER® DMSO Free GMP grade is a chemically defined freezing solution that does not contain DMSO as an anti-freezing agent. It was developed for customers who do not prefer to use DMSO-containing cryopreservation solution due to a variety of their intended uses. STEM-CELLBANKER® DMSO Free GMP grade is also manufactured in compliance with JPN, EU, US, and PIC/S GMP guidelines.

    Key Features

    * This product is U.S. FDA DMF registered. Contact us to request Letter of Authorization submission.

    Technical Information

    Cells Tested

    When tested in the following cells with this product, 80-90% of viability of cells was observed.
    Complete List of Cells Tested [PDF]

    Cell type Description
    129SV Mouse ES cell
    201B7 Human iPS cell
    2D-8 Murine hybridoma
    Caco-2 Human colonic adenocarcinoma cell
    Canine PBMC Canine peripheral blood mononuclear cells
    Feline PBMC Feline peripheral blood mononuclear cells
    HeLa Human uterine cervical carcinoma cell
    HepG2 Human hepatocellular carcinoma cell
    Jurkat Human T-cell line
    K562 Human Caucasian chronic myelogenous leukemia cell
    NBM-Lu Normal newborn murine fibroblast cell line
    P3/x63-Ag8.U1 Murine myeloma cell
    SK007 Human B-cell line
    UE6E7-16 Human Mesenchymal cell
    UE7T-13 Human Mesenchymal Stem cell
    YAC-1 Murine lymphoblast

    Protocol

    Freezing

    For optimum results, cells for cryopreservation should be in log phase of growth. Similar or other standard freezing protocols may be substituted.

    1. Examine and make sure the cell culture is free of contamination, in healthy and at proper confluency.
    2. Perform a cell count to determine the viability of cells.
    3. Centrifuge at 1,000 – 2,000 rpm, 4°C for 3 to 5 minutes to gently pellet the cells. Remove the supernatant with an aspirator.
    4. Gently suspend STEM-CELLBANKER® GMP grade cryopreservation medium (1 mL for 5×10⁵ – 5×10⁶ cells).
    5. Transfer 1 mL of the cell suspension to cryopreservation vial labeled with appropriate information (the cell line name, concentration, passage date etc.).
    6. Place the vials directly in -80℃ for storage.
    7. (OPTIONAL) Transfer the frozen vials to a liquid nitrogen storage tank after the vials have been frozen for at least 24 hours.

    IMPORTANT: Optimum protocol may change with the cell types.

    Thawing

    1. Remove the cryopreservation vial from the freezer and quickly thaw cells in a 37°C shaking water bath or shake by hand.
    2. Transfer the content to a centrifugation tube then immediately dilute and gently mix with 10mL of complete cell culture medium. Using CELLOTION® instead of complete culture medium will prevent adhesion of cells to the wall of the tube, increasing the recovery rate.
    3. Centrifuge cells at 1,000 – 2,000 rpm, 4°C for 3 to 5 minutes. Remove the supernatant with an aspirator.
    4. Gently resuspend the cells with an appropriate volume of complete cell culture medium then plate in a culture flask or plate
    5. Continue the culture procedures according to standard protocols.

    Specification

    • Manufactured By : Zenogen Pharma Co., Ltd
    • Size : 20mL & 100mL
    • Storage and Stability : 2 to 8℃ or below -20℃
    • Expiration date : 3 years after the date of manufacture

    Pricing

    STEM-CELLBANKER®
    DMSO free GMP grade

    20ml size

    Price: $65.00

    100ml size

    Price: $260.00

    20ml size x 4

    Price: $240.00

    * This product is U.S. FDA DMF registered. Contact us to request Letter of Authorization submission.

    References & Literature

    Mao, X. & Zhao, S. Neuronal Differentiation from Mouse Embryonic Stem Cells In vitro. JoVE e61190 (2020) doi: 10.3791/61190.

    Ueda, H. et al. Establishment of in vitro 3D spheroid cell cultivation from human gynecologic cancer tissues. STAR Protocols 2, 100354 (2021) doi: 10.1016/j.xpro.2021.100354.

    Sako, K. et al. Optimization of Spheroid Colony Culture and Cryopreservation of Nucleus Pulposus Cells for the Development of Intervertebral Disc Regenerative Therapeutics. Applied Sciences 11, (2021) doi: 10.3390/app11083309.

    Yagishita, S. et al. Characterization of the large-scale Japanese patient-derived xenograft (J-PDX) library. Cancer Science 112, 2454–2466 (2021) doi: 10.1111/cas.14899.

    Mikłosz, A. et al. Does TBC1D4 (AS160) or TBC1D1 Deficiency Affect the Expression of Fatty Acid Handling Proteins in the Adipocytes Differentiated from Human Adipose-Derived Mesenchymal Stem Cells (ADMSCs) Obtained from Subcutaneous and Visceral Fat Depots? Cells 10, (2021) doi: 10.3390/cells10061515.

    Ueno, K. et al. Freezing of cell sheets using a 3D freezer produces high cell viability after thawing. Biochemistry and Biophysics Reports 28, 101169 (2021) doi: 10.1016/j.bbrep.2021.101169.

    Other Documents

    FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES