BIOMIMESYS® Brain is a Hyaluronic acid (HA) based scaffold 3D cell culture model. This groundbreaking 3D cell culture technology associates the behavior of a solid scaffold and of a hydrogel, which we call “Hydroscaffold”. The Hydroscaffold’s composition and mechanical properties can mimics the microenvironment of brain tissues. It is proven to provide better in vitro/in vivo correlation than 2D models.
Mimics the Microenvironment of Brain Tissue
By mimicking the low stiffness of brain tissues, BIOMIMESYS® Brain allows to grow mini-brains (spheroids of brain cells) or neuronal networks in 3D.
For a better in vitro/in vivo correlation
By using a brain-specific ECM with BIOMIMESYS® Brain, Luhmes cells showed lower sensitivity to Parkinson’s disease inducers in BIOMIMESYS® compared to 2D culture.
Ready to Use
BIOMIMESYS® Oncology is EASY & READY TO USE. Upon receiving the vacuum sealed 96-well plate open it (under a hood) and add the cells directly on top of the matrix. Changing the culture medium is easy as well. To remove medium, simply draw the medium with a pipette between the matrix and the edge of the well. To refresh the medium, place fresh medium onto the surface of the matrix.
Successfully Tested Cells
Human brain metastasis: SA87
Human neuroblastoma : SH-SY5Y
Human glioblastoma : CB109 / CB74 / CB191
Human gliome : DIPG007
Normal human neurons : Luhmes
Normal human colon fibroblast : CCD18-co
Normal mouse preadipocytes : 3T3-L1 / 3T3-F442A
Human colorectal adenocarcinoma : HCT 116
Human liver hepatocellular carcinoma
Human astrocytes : cryopreserved
Mouse neuronal cells
Human induced pluripotent stem cells : CNS4U, Peri4U
Complete List of Tested Cells [PDF]
Compatible with Most Downstream Applications
BIOMIMESYS® hydroscaffold has many properties (transparent, porous, biodegradable, & solid) making it ideal for use with numerous downstream applications.
- Hyaluronic acid (HA) (1.6MDa) grafted with RGDS
- Adipic acid dihydrazide (ADH) as crosslinker
- Collagen Type IV
- cationic bioplolymer
- Young’s modulus: ~0.6kPa – different stiffness is available through customization
- Porosity: 117±23μ
24 Hydrogel / 96 Well
- SKU: BIO_BRA_96_24_transp
- SKU: BIO_BRA_96_24_black
96 Hydrogel / 96 Well
- SKU: BIO_BRA_96_96_transp
- SKU: BIO_BRA_96_96_black
References & Literature
De Conto, V. et al. In vitro differentiation modifies the neurotoxic response of SH-SY5Y cells. Toxicology in Vitro 77, 105235 (2021) doi: 10.1016/j.tiv.2021.105235.
FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES