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    Cline Nano Gradient

    Nano-patterned surfaces for mimicking tissue function

    Product Basics

    Cline Scientific has developed an innovative nanotechnology-based product for work within Life Science (e.g. stem cell and cancer research). It is the only company in the world providing surface gradients of nanoparticle-bound molecules for in-vitro applications. Cline Nano Gradients and Cline Nano Surfaces consist of nano-patterns of gold nanoparticles onto which biomolecules can be attached to create surfaces mimicking tissue function at the molecular level.
    The unique feature of the Cline Nano Gradient is the continuous dispersion of the gold nanoparticles onto a surface in a gradient pattern. This gradient can be utilized to screen for sensitive reactions/interactions between cells and molecules, thus determining the optimal composition of biomolecules responsible for a certain cellular response. The region on the gradient responsible for a reaction can be recreated in a Cline Nano Surface

    Key Benefits

    Technical Information

    Technology

    Cline’s patented method, used to control the average distance between nano particles, is the key technology in creating a continuous density gradient.

    Biomolecule Binding

    There are two sites where your biomolecule of choice can bind to the Cline Nano Gradient:

    • The first location to bind molecules is the nanoparticle gradient itself (Biomolecule A in figures 1 & 2).
    • The second binding-site is the surface space between the nanoparticles (Biomolecule B in figures 1 & 2).

    When both sites are coupled with biomolecules, it forms a double gradient, as shown in figure 2.

    Binding method (examples)

    Biomolecule A Biomolecule B
    Thiol-linker-A
    Thiol-PEG-NeutravidinBiotin-A
    Thiol-NeutravidinBiotin-A
    Electrostatic Binding*
    ** Nanoparticles are positively charged, which allows for electrostatic coupling.

    Applications

    1. ​Stem Cell Differentiation

    “One surface replaces thousands of experiments”
    The immediate environment is critical for stem cells in regulation of their function. The use of Cline Nano Gradient to screen for the exact needs of your cells, allows for faster advances in stem cell R&D. The area in the gradient where optimal cell differentiation is found can then easily be recreated using Cline Nano Surface.

    Benefits
    pilelabs
    1
    Seed Cells

    Seed your cells on a Cline Nano Gradient of surface- bound molecules.

    pilelabs
    2
    Analyze and Select

    Allow the cells to react to their surroundings and then identify your optimal surface composition.

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    3
    Reproduce

    The Cline Nano Surface recreates the optimal conditions for your cells and allows you to reproduce your results.

    2. Cencer Research & Cell Migration

    “One surface replaces thousands of experiments”
    “Ultimate cell control through molecular precision” 
    The Cline Nano Gradient can be utilized to study sensitive gradual cell behaviors such as cellular movement and cell migration. This is achieved by creating a surface gradient of biomolecules stimulating cellular movement. Cells are cultured, then analyzed by live imaging or by staining.

    Benefits
    pilelabs
    1
    Seed Cells

    Seed your cells on a Cline Nano Gradient of surface bound biomolecules.

    pilelabs
    2
    Migration

    Study how the cells migrate on the surface gradient of biomolecules.

    pilelabs
    3
    End Point

    Quantify cell migration according to your preferred method (e.g. cell numbers, motility, viability, or distance migrated)

    Specification

    • Format: Glass Cover slip
    • Manufactured by: Cline Scientific

    Pricing

    Cline Nano Gradient

    This product is custom-made only.
    Please contact us for a quote.

    References & Literature

    Lundgren, Anders et al. “Gold-nanoparticle-assisted self-assembly of chemical gradients with tunable sub-50 nm molecular domains.” Particle & Particle Systems Characterization 31 (2014): 209-218. https://doi.org/10.1002/ppsc.201300154

    Hulander, Mats et al. “Immune complement activation is attenuated by surface nanotopography.” International journal of nanomedicine vol. 6 (2011): 2653-66. https://doi.org/10.2147/IJN.S24578

    Hulander, M et al. “Gradients in surface nanotopography used to study platelet adhesion and activation.” Colloids and surfaces. B, Biointerfaces vol. 110 (2013): 261-9. https://doi.org/10.1016/j.colsurfb.2013.04.010

    FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES