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    Optimal Cell Preservation with CELLBANKER® 2: Zenogen Pharma’s Ready-to-Use, Serum-Free Cryoprotectant. Suitable for Antibody/Protein Production Cells.

    A flagship of the renowned CELLBANKER® series, a part of Japan’s most trusted line of cryopreservation media which maintains High Viability after years of preservation.

    CELLBANKER® 2 is an excellent option if you’re considering an alternative for graduating from a traditional serum-based cryoprotectant but do not require clinical/GMP grade.

    Crafted with a specialized formula to cater to a wide array of mammalian cell cultures, CELLBANKER® 2 stands out as the ultimate solution for peptide/protein expressing cells.

    Experimental results (Click to Expand)
    CellsPreservation period (Year)Viability of cells (%)
    Preserved at -80℃
    EBV transformed cell590
    List of Cells Tested [PDF] – Cell types are constantly being added.

    With its unique formulation, CELLBANKER® 2 guarantees robust cryopreservation and preserves exceptional viability through the freeze-thaw process. Moreover, it effectively eliminates concerns about serum contamination, ensuring reliable cell storage for your research needs.

    Protocol (Click to Expand)


    For optimum results, cells for cryopreservation should be in log phase of growth. Similar or other standard freezing protocols may be substituted.

    1. Examine and make sure the cell culture is free of contamination, in healthy and at proper confluency.
    2. Perform a cell count to determine the viability of cells.
    3. Centrifuge at 1,000 – 2,000 rpm, 4°C for 3 to 5 minutes to gently pellet the cells. Remove the supernatant with an aspirator.
    4. Gently suspend CELLBANKER® 2 cryopreservation medium (1 mL for 5×10⁵ – 5×10⁶ cells).
    5. Transfer 1 mL of the cell suspension to cryopreservation vial labeled with appropriate information (the cell line name, concentration, passage date etc.).
    6. Place the vials directly in -80℃ for storage.
    7. (OPTIONAL) Transfer the frozen vials to a liquid nitrogen storage tank after the vials have been frozen for at least 24 hours.
    Procedure for Use


    1. Remove the cryopreservation vial from the freezer and quickly thaw cells in a 37°C shaking water bath or shake by hand.
    2. Transfer the content to a centrifugation tube then immediately dilute and gently mix with 10mL of complete cell culture medium. Using CELLOTION® instead of complete culture medium will prevent adhesion of cells to the wall of the tube, increasing the recovery rate.
    3. Centrifuge cells at 1,000 – 2,000 rpm, 4°C for 3 to 5 minutes. Remove the supernatant with an aspirator.
    4. Gently resuspend the cells with an appropriate volume of complete cell culture medium then plate in a culture flask or plate
    5. Continue the culture procedures according to standard protocols.

    Customization Options Available on Request.

    You can confidently rely on CELLBANKER® 2 for dependable and efficient cell preservation, enhancing your research and biotechnological pursuits.

    Publications (Click to Expand)

    Rimland, C. A. et al. Regional Differences in Human Biliary Tissues and Corresponding In Vitro–Derived Organoids. Hepatology 73, 247–267 (2021) doi: 10.1002/hep.31252
    (Liver tissue and organoids, CELLBANKER 2)

    Inui, J., Ueyama-Toba, Y., Mitani, S. & Mizuguchi, H. Development of a method of passaging and freezing human iPS cell-derived hepatocytes to improve their functions. PLOS ONE 18, e0285783 (2023) doi: 10.1371/journal.pone.0285783
    (iPS derived.Hepatocyte-like cell, Compared CELLBANKER 1, STEM-CELLBANKER and CELLBANKER 2)

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