PeptiGrowth PG-011
FGF2 Alternative Peptide (FGFR1c agonist)
Product Basics
Fibroblast Growth Factor 2 (FGF2/basic FGF) binds to FGFR1c and activates intracellular signaling and is essential for maintaining the undifferentiated state of iPSCs in culture.
PG-011 is an alternative to FGF2 that binds FGFR1c and exhibits agonist activity toward FGFR1c, demonstrating equivalent efficacy to recombinant FGF2 at 1/3 of the concentration. Unlike recombinant FGF2, known for its instability, PG-011 is highly stable, retaining its activity even after 4 days at 37℃ in culture media. It also exhibits activity against various cell types, such as iPSCs and muscle satellite cells, and shows cross-reactivity with bovine cells, making it a potential application not only in regenerative medicine and cell therapy but also in the production of cultured meat.
Key Features
- Alternative to FGF2
- Binds FGFR1c and exhibits agonist activity toward FGFR1c
- Comparable activity at 1/3 of the concentration of rFGF2*
- Exhibits activity against various cell types such as iPSC, muscle satellite cells
- Cross-reactive with bovine cells, supporting cultured meat application
- Highly stable. ~100% remained after 4 days in serum-free media at 37˚C
- Fully synthetic and animal-free
GMP grade can be manufactured upon request.
*Please note that this figure is an example and concentration optimization is needed.
Technical Information
(1) PG-011 demonstrated comparable proliferation activity to FGF2
PeptiGrowth Inc. conducted a comparison of the cell proliferation activity of PG-011 and FGF2 using bone marrow-derived human MSCs (mesenchymal stem cells). The results showed that PG-011 exhibited comparable cell proliferation activity to FGF2. When compared on a mass concentration basis (ng/mL), PG-011 demonstrated equivalent activity to FGF2 at approximately one-third of the concentration.
(2) iPSCs Pluripotency Maintenance Ability
i. Morphology
PeptiGrowth Inc. evaluated the proliferation and pluripotency maintenance abilities of iPSCs using PG-011. The culture media were supplemented with either FGF2 (100 ng/mL, approx. 5.9 nM) or an equivalent molar concentration of PG-011 (approx. 30 ng/mL). After 7 days of culture, subculturing was performed. Following three rounds of subculturing, the morphology of the iPSCs was observed under a microscope. The results confirmed that iPSCs cultured with PG-011 exhibited a morphology similar to that of cells cultured with FGF2.
ii. Undifferentiated marker measurements
After the third round of subculturing, iPSCs were collected on day 7 of culture, and the percentage of positive cells for pluripotency markers (SSEA4, TRA-1-60) was measured using flow cytometry to assess the pluripotent state of the iPSCs. The results confirmed that, for all three cell lines evaluated, PG-011 and FGF2 showed similar rates of positive pluripotency markers. This confirms that PG-011 has equivalent iPSC pluripotency maintenance capability to FGF2.
(3) Superior Stability
i. Comparison of the stability compared to recombinant FGF2
PeptiGrowth Inc. compared the stability of PG-011 and FGF2. Each sample was incubated at 37˚C in serum-free medium for 1 day and 4 days. After incubation, the samples were evaluated for their proliferation activity on MSCs (concentration condition: 0.06nM). The results showed that while FGF2 exhibited a decrease in activity over the incubation time, PG-011 maintained its activity even after 4 days of incubation. This indicates that PG-011 possesses superior stability compared to FGF2.
ii. Stability in culture environment with HULEC cells
(4) Proliferation Activity on Bovine-Derived Muscle Satellite Cells
Results from the proliferation assay using PG-011 on bovine-derived muscle satellite cells demonstrated that PG-011 exhibited proliferation activity even at a concentration of 0.2ng/mL. This suggests that PG-011 has potential applications not only in regenerative medicine and cell therapy but also in the production of cultured meat.
Specification
- Storage temperature: -20°C (Ship at 4°C)
- Shelf life: 2 years (To be extended)
- Manufactured by: PeptiGrowth Inc.
Pricing
100ug
- SKU: PG-011-100ug
- Size: 100ug (eq. ~300ug of rFGF2)
- Price: $230.00
1mg
- SKU: PG-011-1mg
- Size: 1mg (eq. ~3mg of rFGF2)
- Price: $1,450.00
10mg
- SKU: PG-011-10mg
- Size: 10mg (eq. ~30mg of rFGF2)
- Price: $8,700.00
References
Coming soon.
Other Documents
FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES