PeptiGrowth PG-010
TPO Alternative Peptide (TPOR agonist)
Product Basics
Thrombopoietin (TPO) stimulates the proliferation and maturation of megakaryocytes and is commonly used for the differentiation of iPSCs into hematopoietic stem cells and manufacturing of allogeneic cell therapy products, such as CAR–T cells.
PG-010 is an alternative to TPO that binds to the thrombopoietin receptor (TPOR) and functions as an epidermal growth factor substitute. It is confirmed to activate ERK in the TPO signaling pathway, as well as the JAK2-STAT5 signaling pathway, similar to TPO. It exhibits agonistic activity equivalent to recombinant TPO and shows comparable activity at 1/2.6 of the concentration of recombinant TPO due to a difference in molecular weight
Key Features
- Alternative to TPO
- Binds to the thrombopoietin receptor (TPOR) and functions as a epidermal growth factor substitute
- Confirmed to activate ERK through both the TPO and JAK2-STAT5 signaling pathwaysComparable activity at 1/2.6 of the concentration of rTPO*
- Fully synthetic and animal-free
GMP grade can be manufactured upon request.
*Please note that this figure is an example and concentration optimization is needed.
Technical Information
(1) PG-010 demonstrated activity nearly equivalent to TPO
Recombinant TPO and PG-010 activities were compared using ERK phosphorylation and reporter gene activation under JAK2-STAT5 signaling pathway. The results indicated that PG-010 demonstrated activity nearly equivalent to TPO on a molar basis, showing similar activity at 2.6 times less concentrations than recombinant TPO based on mass concentration (ng/mL).
(2) Differentiation of iPSCs into NKT cells through hematopoietic stem and progenitor cells (HSPCs), using PG peptides (PG-007, 008 and 010) or using VEGF, CHIR99021 (Wnt3a agonist), and TPO.
PeptiGrowth
Control
PeptiGowth Inc. demonstrated the differentiation of iPSCs into NKT cells through differentiation into hematopoietic stem and progenitor cells (HSPCs) using PG-007, PG-008, and PG-010, and compared the productivity of HSPCs and NKT cells and the functionality of NKT cells with the control condition using VEGF, CHIR99021 (Wnt3a agonist), and TPO.
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Specification
- Storage temperature: -20°C (Ship at 4°C)
- Shelf life: 2 years (To be extended)
- Manufactured by: PeptiGrowth Inc.
Pricing
10ug
- SKU: PG-010-10ug
- Size: 10ug (eq. ~26ug of rTPO)
- Price: $300.00
100ug
- SKU: PG-010-100ug
- Size: 100ug (eq. ~260ug of rTPO)
- Price: $1,550.00
1mg
- SKU: PG-010-1mg
- Size: 1mg (eq. ~2600ug of rTPO)
- Price: $8,900.00
References
Coming soon.
FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES