iMatrix 511 Series
Maintenance and expansion of pluripotent stem cells (PSCs) & mesenchymal stem cells (MSCs)
Product Basics
The iMatrix-511 is an innovative cell culture matrix that is compatible with a wide variety of cell types. It is exceptionally well-suited for pluripotent and mesenchymal stem cells. This product consists of recombinant Laminin-511 E8 protein fragments, which allow ES/iPS cells and MSCs to be maintained in xeno-free culture conditions. It also enables the passaging of single cells and provides greater adhesion than full-length Laminin, Vitronectin, or Matrigel® (Miyazaki et al., Nature Commun. 3; 1236 (2012)).
Key Features
*Available in three grades, including GMP, to support a smooth transition from R&D to clinical stages
Advantages vs. Matrigel®
- Defined xeno-free culture
- Expansion efficiency is ~200x higher
- Work is simplified for reduced labor and human error
- Cost: $1–2 per well (iMatrix-511) vs. $3–4 per well (Matrigel®)
Greater Cell Adhesion & Proliferation
Human iPS cells exhibit stronger adhesion to the Laminin-511 E8 fragment than to the “Laminin-511 (Intact).”
The adhesion strength of the Laminin-511 E8 fragment and other cell culture substrates to human iPS cells was compared. Human iPS cells adhered more strongly to the Laminin-511 E8 fragment than to “Laminin-511 (Intact).”
Absorbance (OD570) represents the relative number of attached cells and was normalized to the maximum response observed on Matrigel®, which was arbitrarily set to 1.
In the same study mentioned above, human ES cells were passaged on Laminin-511 E8 fragment and other cell culture substrates. When human ES cell colonies were dispersed individually at the time of passage, it was observed that single cells adhered rapidly to Laminin-511 E8 and proliferated immediately.
LM-E8s are more effective for single-cell culture than Matrigel®, fibronectin, or vitronectin
Culture more ES/iPS cells using Laminin-511 E8 fragment
The graph above compares the numbers of ES/iPS cells cultured by the conventional method (colony) for 30 days with those cultured using the Laminin-511 E8 fragment. The results confirm that the cell count increased by at least 200 times when the Laminin-511 E8 fragment was employed.
Technical Information
Single-Cell Passaging Ready
With conventional methods, ES/iPS cells perish when dispersed into single cells, necessitating the maintenance of a cell colony during passaging.
Using the Laminin-511 E8 fragment as a cell culture matrix enables the culturing of single ES/iPS cells, which in turn reduces the time required to optimize culture methods and drastically enhances the efficiency of cell cultures.
Pre-Mix Method (Without Pre-Coating) For Higher Efficiency
Advantages
- Requires half the amount of iMatrix protein needed compared to pre-coating method
- Lower preparation time (no incubation needed)
- No need for spare pre-coated plates
- Prevents uneven growth due to irregular coating
Comparison of iMatrix-511, laminin-521, and recombinant human vitronectin in supporting cell adhesion using pre-coat versus pre-mix methods. The pre-mix method reduces the amount of substrate needed for maximum adhesion of hPSCs, while the iMatrix-511 pre-coating method still supports robust cell growth even with smaller amounts of substrate.
Scraper-Free Cell Detachment Using iMatrix
Easy iMatrix
Ready-to-use Easy iMatrix‑511 provides a reliable Laminin‑511 E8 coating for efficient feeder-free cell culture workflows.
- Ready-to-use: eliminates dilution or mixing
- Pre-diluted at a concentration of 1.6ug/mL
- Simplifies workflows and reduces hands-on preparation
- Ensures consistent, reproducible coating results
Specification
- Format: Liquid Solution (175 ug/vial @0.5 mg/mL), Solvent: PBS (-)
- Storage temperature: 2-15 °C
- Manufactured by : Nippi, Inc. 1-1-1 Senju-Midoricho, Adachi-ku, Tokyo, Japan
- Available in 3 grades:
- iMatrix-511: expressed with CHO-S Cell
- iMatrix-511 SILK: cost-effective version expressed with transgenic silkworm cocoon
- iMatrix-511MG: Clinical-grade (GMP) [Please inquire for more details]
Pricing
iMatrix-511
- Expressed by CHO-S Cell
- Liquid Solution (175 ug/vial @0.5 mg/mL), Solvent: PBS (-)
- 175ug coats roughly 2-6 six well plates via conventional method
- SKU: N-892013
- Price: $80.00
- Incl. US (Continental) shipping fee
- * Only one purchase per lab *
350ug size (175ug x 2 tubes)
- SKU: N-892011
- Price:
$309.00→ $216.00
1050ug size (175ug x 6 tubes)
- SKU: N-892012
- Price:
$725.00→ $590.00
iMatrix-511 Silk
- Expressed by transgenic silkworm cocoon
- Cost-effective alternative to the standard iMatrix-511
- Liquid Solution (175 ug/vial @0.5 mg/mL), Solvent: PBS (-)
- 175ug coats roughly 2-6 six well plates via conventional method
- SKU: N-892022
- Price: $80.00
- Incl. US (Continental) shipping fee
- * Only one purchase per lab *
Easy iMatrix-511
- Pre-diluted at a concentration of 1.6ug/mL
- Ready to Use
- 100mL = roughly 16 6-well plate (amount to coat well surface = 0.25ug/cm²)
Easy iMatrix-511 (CHO-S Cell)
- SKU: N-892019
- Price: $80.00
- Incl. US (Continental) shipping fee
- * Only one purchase per lab *
- SKU: N-892018
- Price: $380.00
Easy iMatrix-511 Silk (Transgenic silkworm cocoon)
- SKU: N-892025
- Price: $80.00
- Incl. US (Continental) shipping fee
- * Only one purchase per lab *
- SKU: N-892024
- Price: $330.00
References & Literature
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Other Documents
- Protocol iMatrix-511
- Protocol iMatrix-511-Silk
- Protocol Easy iMatrix-511/iMatrix-511-Silk
- MSDS iMatrix-511
- MSDS iMatrix-511-Silk
- MSDS Easy-iMatrix-511
- MSDS Easy-iMatrix-511-Silk
- ECM Pre-mix method
- Detachment Method using EDTA for human iPS cells
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