iMatrix 411
Induction of vascular endothelial cells from human PSCs
Product Basics
iMatrix-411 is a recombinant Laminin-411 E8 fragment protein used as a cell culture matrix. Laminin-411 is a predominant isoform found in the basal membrane of the endothelial cells in capillary vessels, and it mainly binds to the cell surface transmembrane receptors integrin α6β1 and α71β1.¹ The E8 fragment maintains full binding activity toward integrin, yet lacks binding activities to other components. The process of switching defined matrices is a critical tool for steering cells towards their intended fate.² Studies have demonstrated that switching the extracellular matrix (ECM) from laminin-511 E8 fragment (iMatrix-511) to laminin-411 E8 during differentiation efficiently induces endothelial progenitor cells from iPS cells.
¹ Nishiuchi, R. et al. Matrix Biol. 25, 189-197-,2006
² Ohta R et al., Scientific Reports, 6(35680), 1-12, 2016
Key Features
- Switching from laminin-511 E8 to laminin-411 E8 during differentiation induces endothelial progenitor cells
- Highly purified and refined human laminin-411 E8 fragment expressed by CHO-S cells
- Feeder-free cell culture
Technical Information
Phenotypes of endothelial cells induced from pluripotent stem cells on iMatrix-411 coated plate
Specification
- Format: Liquid Solution (175 ug/vial @0.5 mg/mL), Solvent: PBS (-)
- Storage temperature: 2-15 °C
- Manufactured by: Nippi, Inc. 1-1-1 Senju-Midoricho, Adachi-ku, Tokyo, Japan
Pricing
iMatrix-411
- Expressed by CHO-S Cell
- Liquid Solution (175 ug/vial @0.5 mg/mL), Solvent: PBS (-)
- Recommended starting concentration: 0.5µg/cm²
- SKU: N-892043
- Price: $80.00
- Incl. US (Continental) shipping fee
- * Only one purchase per lab *
350ug (175ug x 2 tubes)
- SKU: N-892041
- Price: $495.00
1050ug (175ug x 6 tubes)
- SKU: N-892042
- Price: $1,350.00
References & Literature
Nishiuchi, R. et al. Ligand-binding specificities of laminin-binding integrins: A comprehensive survey of laminin–integrin interactions using recombinant α3β1, α6β1, α7β1 and α6β4 integrins. Matrix Biology 25, 189–197 (2006) doi: 10.1016/j.matbio.2005.12.001.
Ohta, R. et al. Laminin-guided highly efficient endothelial commitment from human pluripotent stem cells. Scientific Reports 6, 35680 (2016) doi: 10.1038/srep35680.
Takayama, K. et al. Laminin 411 and 511 promote the cholangiocyte differentiation of human induced pluripotent stem cells. Biochemical and Biophysical Research Communications 474, 91–96 (2016) doi: 10.1016/j.bbrc.2016.04.075.
Tang, J. & Saito, T. A Novel Fragment Derived from Laminin-411 Facilitates Proliferation and Differentiation of Odontoblast-Like Cells. BioMed Research International 2018, 9465383 (2018) doi: 10.1155/2018/9465383.
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FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES