iMatrix 332
Maintenance and purification of corneal epithelial cells and keratinocytes.
Product Basics
iMatrix-332 is a recombinant human Laminin 332-E8 fragments protein expressed by CHO-S cells. Laminin 332 is composed of α3, β2, and γ2 chains, which are known to bind to integrins α3β1 and α6β4. It is essential for cells in the epithelial basement membranes, lining the surfaces of the body such as the skin, hair follicles, oral cavity, gastrointestinal and urinary tracts, lungs, and various glands. It is an important component of the “skin basement membrane” that exists between the epithelium and dermis of the skin and is also expressed in human retinal pigment epithelium (RPE) and hair follicle cells.
Key Features
- Highly purified and refined human laminin-332 E8 fragment expressed by CHO-S cell
- iMatrix-332 can be used to support directed differentiation for corneal epithelial cells and keratinocytes
- Feeder-free cell culture
Applications
iMatrix-332 can be used as cell culture matrix for various cell types including ES/iPS cells.
Procedure
- Dilute the solution with sterile PBS (-). Coat dishes with 0.5 ug/cm². Add 2 mL of diluted iMatrix-332 solution to the well. The optimal coating concentration depends on cell lines from 0.1 to 1.5 ug/cm². (For example, when you use 6-well plate (9.6 cm²/well), add 9.6 ul of iMatrix-332 (4.8 ug) in 1.99 ml of PBS(-).)
- Incubate for 1 hour at 37 °C, 3 hours at room temperature, or over-night at 4 °C.
- Remove remaining fluid from the coated surface. No rinse is needed.
- Immediately plate the cells at desired density.
Notes: Don’t allow the plate to dry. Briefly spin down all liquid in the tube before use. Avoid repeated freeze-thaw cycles.
Specification
- Product size: 1050ug (175ug x6pcs), 350ug (175ug x2pcs), Sample (175ug x1)
- Format: Liquid Solution
- Concentration: 175ug/vial @ 0.5mg/mL (± 10%))
- Solvent: 20mM Phosphate Buffer, 500mM NaCl
- Storage temperature: 2-15 °C
- Shelve life: 2 yrs from manufacturing date.
- Manufactured by: Nippi, Inc.
Pricing
iMatrix-332
- Expressed by CHO-S Cell
- Liquid Solution (175 ug/vial @0.5 mg/mL), Solvent: PBS (-)
- Recommended starting concentration: 0.5µg/cm²
- SKU: N-892033
- Price: $80.00
- Incl. US (Continental) shipping fee
- * Only one purchase per lab *
350ug (175ug x 2 tubes)
- SKU: N-892031
- Price: $495.00
1050ug (175ug x 6 tubes)
- SKU: N-892032
- Price: $1,350.00
References & Literature
Nishiuchi, R. et al. Ligand-binding specificities of laminin-binding integrins: A comprehensive survey of laminin–integrin interactions using recombinant α3β1, α6β1, α7β1 and α6β4 integrins. Matrix Biology 25, 189–197 (2006) doi: 10.1016/j.matbio.2005.12.001.
Miyazaki, T. et al. Laminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotent stem cells. Nature Communications 3, 1236 (2012) doi: 10.1038/ncomms2231.
Shibata, S. et al. Selective Laminin-Directed Differentiation of Human Induced Pluripotent Stem Cells into Distinct Ocular Lineages. Cell Reports 25, 1668-1679.e5 (2018) doi: 10.1016/j.celrep.2018.10.032.
Shibata, S. et al. Cell-Type-Specific Adhesiveness and Proliferation Propensity on Laminin Isoforms Enable Purification of iPSC-Derived Corneal Epithelium. Stem Cell Reports 14, 663–676 (2020) doi: 10.1016/j.stemcr.2020.02.008.
Skowron-Kandzia, K. et al. Gene Expression in Amnion-Derived Cells Cultured on Recombinant Laminin 332—A Preliminary Study. Frontiers in Medicine 8, (2021).
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FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES