iMatrix-111
Maintenance and purification of HPCs, Hepatocytes and Nerve cells.
Product Basics
iMatrix-111 is a highly purified recombinant human Laminin 111-E8 fragment expressed by CHO-S cells. Laminin 111 is known to be present in the liver and binds to integrins α7X2β1 and α6β1. It is essential for the formation of the three embryonic germ layers by pluripotent cells in the inner cell mass. Laminin 111 supports the survival, proliferation, and differentiation of many different cell types in vitro. It is also known to co-signal with various growth factors in a cell type-dependent manner, which may explain the major biological effects that Laminin 111 exerts on a wide selection of cell types.
Key Features
- Highly purified and refined human laminin-111 E8 fragment expressed by CHO-S cell
- Used to support directed differentiation of HPCs, Hepatocytes and Nerve cells
- Feeder-free cell culture
Applications
iMatrix-111 can be used as cell culture matrix for various cell types including ES/iPS cells.
Procedure
- Dilute the solution with sterile PBS (-). Coat dishes with 0.5 ug/cm². Add 2 mL of diluted iMatrix-111 solution to the well. The optimal coating concentration depends on cell lines from 0.1 to 1.5 ug/cm². (For example, when you use 6-well plate (9.6 cm²/well), add 9.6 ul of iMatrix-111 (4.8 ug) in 1.99 ml of PBS(-).)
- Incubate for 1 hour at 37 °C, 3 hours at room temperature, or over-night at 4 °C.
- Remove remaining fluid from the coated surface. No rinse is needed.
- Immediately plate the cells at desired density.
Notes: Don’t allow the plate to dry. Briefly spin down all liquid in the tube before use. Avoid repeated freeze-thaw cycles.
Specification
- Product size: 1050ug (175ug x6pcs), 350ug (175ug x2pcs), Sample (175ug x1)
- Format: Liquid Solution
- Concentration: 175ug/vial @ 0.5mg/mL (± 10%))
- Solvent: 20mM Phosphate Buffer, 250mM NaCl
- Storage temperature: 2-15 °C
- Shelve life: 2 years from the date of manufacture
- Manufactured by: Nippi, Inc.
Pricing
iMatrix-111
- Expressed by CHO-S Cell
- Liquid Solution (175 ug/vial @0.5 mg/mL), Solvent: PBS (-)
- Recommended starting concentration: 0.5µg/cm²
- SKU: N-892073
- Price: $80.00
- Incl. US (Continental) shipping fee
- * Only one purchase per lab *
350ug (175ug x 2 tubes)
- SKU: N-892071
- Price: $495.00
1050ug (175ug x 6 tubes)
- SKU: N-892072
- Price: $1,350.00
References & Literature
Taniguchi, Y. et al. The C-terminal region of laminin beta chains modulates the integrin binding affinities of laminins. J Biol Chem 284, 7820–7831 (2009) doi: 10.1074/jbc.M809332200.
Doi, D. et al. Isolation of Human Induced Pluripotent Stem Cell-Derived Dopaminergic Progenitors by Cell Sorting for Successful Transplantation. Stem Cell Reports 2, 337–350 (2014) doi: 10.1016/j.stemcr.2014.01.013.
Takayama, K. et al. Generation of safe and therapeutically effective human induced pluripotent stem cell-derived hepatocyte-like cells for regenerative medicine. Hepatology Communications 1, 1058–1069 (2017) doi: 10.1002/hep4.1111.
Takayama, K. et al. Enrichment of high-functioning human iPS cell-derived hepatocyte-like cells for pharmaceutical research. Biomaterials 161, 24–32 (2018) doi: 10.1016/j.biomaterials.2018.01.019.
Kiyozumi, D., Nakano, I., Sato-Nishiuchi, R., Tanaka, S. & Sekiguchi, K. Laminin is the ECM niche for trophoblast stem cells. Life Sci. Alliance 3, e201900515 (2020) doi: 10.26508/lsa.201900515.
Guo, G. et al. Human naive epiblast cells possess unrestricted lineage potential. Cell Stem Cell 28, 1040-1056.e6 (2021) doi: 10.1016/j.stem.2021.02.025.
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FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES