Iwai North America Inc.

541 Taylor Way Suite# 4
 San Carlos, CA 94070
 Phone : (650) 486-1541
Fax : (650) 394-8638
 Open weekdays 9 AM-6 PM (PST)
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iMatrix-411
Catalog# and Pricing

**For Research Use Only. Not For Use in Diagnostic Procedures**  

iMatrix-411 – Expressed by CHO-S Cell
- Format: Solution (175ug/vial @0.5mg/mL) Solvent: PBS(-)) 
- 1mg of iMatrix-411 is enough to coat more than 35 6-well plates for normal use
- Recommended concentration: 0.5µg/cm²
Datasheet & MSDS

* Cat# N-892041 : Size 350ug (175ug x 2 tubes)
---- List Price: $435.00

* Cat# N-892042 : Size 1050ug (175ug x 6 tubes)  
---- List Price: $1,160.00

* Cat# N-892043 : Trial size (175ug x 1 tube) -- One time purchase only. 
----- List Price: $80.00 (Incl. US Domestic Shipping Fee)



Reference Literature

- Takayama K. et al., Laminin 411 and 511 promote the cholangiocyte differentiation of human induced pluripotent stem cells. Biochemical and biophysical research communications, 474 (1), 91-96, 2016

- Ohta R et al., Laminin-guided highly efficient endothelial commitment from human pluripotent stem cells. Scientific Reports, 6 (35680), 1-12, 2016

Product Basics:

iMatrix-411 is a recombinant Laminin-411 E8 fragment protein used as a cell culture matrix. Laminin-411 is a major isoform that lines the basal membrane of the endothelial cells in capillary vessels and binds mainly to the cell surface transmembrane receptors integrin α6β1 and α71β1.¹ The E8 fragment retains full binding activity toward integrin but lacks binding activities to other components. The switching of defined matrices is an important tool for guiding cells towards their desired fate.² Studies have shown that changing the ECM from laminin-511 E8 fragment (iMatrix-511) to laminin-411 E8 during differentiation efficiently induces endothelial progenitor cells from iPS cells.  
¹ Nishiuchi, R. et al. Matrix Biol. 25, 189-197, 2006
² Ohta R et al., Scientific Reports, 6(35680), 1-12, 2016
Red: acetyl-LDL uptake ///// Green: CD31 expression ///// Blue: nuclear staining 

Scale bar: 10uM                  (Ohta R et al., Scientific Reports, 6(35680), 1-12, 2016) 



FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES

Manufactured by : 
Nippi, Inc.
1-1-1 Senju-Midoricho, Adachi-ku, Tokyo, Japan

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Matrix for Cell Culture

    Key Features:       

    - Switching from laminin-511 E8 to laminin-411 E8 during differentiation induces endothelial progenitor cells

    - Highly purified and refined human laminin-411 E8 fragment expressed by CHO-S cells

    - Feeder-free cell culture

Phenotypes of endothelial cells induced from pluripotent stem cells on iMatrix-411 coated plate
Endothelial cells derived from human ES cell line KhES-1
Endothelial cells derived from human iPS cell line 253G4