Iwai North America Inc.

541 Taylor Way Suite# 4
 San Carlos, CA 94070
 Phone : (650) 486-1541
Fax : (650) 394-8638
 Open weekdays 9 AM-6 PM (PST)
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iMatrix-511 is an innovative cell culture matrix compatible with a wide variety of cell types, and exceptionally well suited for pluripotent stem cells. This product is comprised of recombinant Laminin-511 E8 protein fragments which permit ES/iPS cells to be maintained in xeno-free culture conditions, enable the passaging of single cells, and provide greater adhesion than full-length Laminin, Vitronectin or Matrigel¹.
 



With the conventional method, ES/iPS cells die when dispersed into single cells, making it necessary to maintain a cell colony when passaging.
Laminin-511 is a major component of the basement membrane used as a scaffold for pluripotent stem cells (ES/iPS cells), as it binds to integrin on cell surfaces. However Laminin-511 is a large protein (800kDa) composed of three chains (α-, β-, and γ-) forming a supramolecular aggregate, making it difficult to produce recombinantly. In order to overcome this challenge, Laminin-511 proteins were fragmented to find the smallest integrin-binding component and the E8 fragment was identified as the most promising result. In fact, hES cells were found to adhere more strongly to the E8 fragment than to the full-length protein.

Matrix for Cell Culture
iMatrix-511
Fig. 1
Product Basics & Structure
Catalog# and Pricing
Reference Literature
- Hayashi, R., Ishikawa, Y., Katori, R., Sasamoto, Y., Taniwaki, Y., Takayanagi, Tsujikawa, M., Sekiguchi, K., Quantock, A. J., Nishida, K. . Coordinated generation of multiple ocular-like cell lineages and fabrication of functional corneal epithelial cell sheets from human iPS cells. Nature Protocols, 12(4), 683-696, (2017).

- Kikuchi, T., Morizane, A., Okita, K., Nakagawa, M., Yamakado, H., Inoue, H., Takahashi, R., Takahashi, J. . Idiopathic Parkinson’s disease patient‐derived induced pluripotent stem cells function as midbrain dopaminergic neurons in rodent brains. Journal of Neuroscience Research, 2017.

- Miyazaki, T., Isobe, T., Nakatsuji, N., & Suemori, H. . Efficient Adhesion Culture of Human Pluripotent Stem Cells Using Laminin Fragments in an Uncoated Manner. Scientific Reports, 7(41165), 1-8(2017).

- Musah, S., Mammoto, A., Ferrante, C. T., Jeanty, S.S., Hirano-Kobayashi, M., Mammoto, T., Roberts, K., Chung, S., Novak, R., Ingram, M., Fatanat-Didar, T., Koshy, S., Weaver, C. J., Church, M. G., Ingber, F. D.. Mature induced-pluripotent-stem-cell-derived human podocytes reconstitute kidney glomerular-capillary-wall function on a chip. Nature Biomedical Engineering, 1, 0074, 2017. 

- Samata, B., Doi, D., Nishimura, K., Kikuchi, T., Watanabe, A., Sakamoto, Y., Kakuta, J., Ono, Y., & Takahashi, J. . Purification of functional human ES and iPSC-derived midbrain dopaminergic progenitors using LRTM1. Nature Communications, 7(13097), 1-11 (2016). 

- Hayashi, R., Ishikawa, Y., Sasamoto, Y., Katori, R., Nomura, N., Ichikawa, T., Araki, S., Soma, T., Kawasaki, S., Sekiguchi, K., Tsujikawa, M., Nishida, K., & Quantock, A. J.. Co-ordinated ocular development from human iPS cells and recovery of corneal function. Nature, 531(7594), 376-380 (2016).

- Matsuno, K., Mae, S. I., Okada, C., Nakamura, M., Watanabe, A., Toyoda, T., Uchida, E., Osafune, K. . Redefining definitive endoderm subtypes by robust induction of human induced pluripotent stem cells.Differentiation; research in biological diversity, (2016).

- Nishimura, K., Doi, D., Samata, B., Murayama, S., Tahara, T., Onoe, H., & Takahashi, J. . Estradiol Facilitates Functional Integration of iPSC-Derived Dopaminergic Neurons into Striatal Neuronal Circuits via Activation of Integrin α5β1. Stem cell reports, 6(4), 511-524 (2016).

- Takayama, K., Mitani, S., Nagamoto, Y., Sakurai, F., Tachibana, M., Taniguchi, Y., Sekiguchi, K., Mizuguchi, H. . Laminin 411 and 511 promote the cholangiocyte differentiation of human induced pluripotent stem cells. Biochemical and biophysical research communications, 474(1), 91-96 (2016).

- Hayashi, Y., Caboni. L., Das, D., Yumoto, F., Clayton, T., Deller, M.C., Nguyen, P., Farr, C.L., Chiu, H.J., Miller, M.D., Elsliger, M.A., Deacon, A.M., Godzik, A., Lesley, S.A., Tomoda, K., Conklin, B.R., Wilson, I.A., Yamanaka, S., Fletterick, R.J.. Structure-based discovery of NANOG variant with enhanced properties to promote self-renewal and reprogramming of pluripotent stem cells. PNAS, vol. 112 no. 154666–4671, (2015)

- Okumura, N., Kakutani, K., Numata, R., Nakahara, M., Schlötzer-Schrehardt, U., Kruse, F., Kinoshita. K., Koizumi, N. . Laminin-511 and-521 Enable Efficient In Vitro Expansion of Human Corneal Endothelial CellsLaminin-511 and-521 Enable Expansion of HCECs. Investigative ophthalmology & visual science, 56(5), 2933-2942 (2015).

- Sasaki, K., Yokobayashi, S., Nakamura, T., Okamoto, I., Yabuta, Y., Kurimoto, K., Ohta, H., Moritoki, Y., Iwatani, C., Tsuchiya, H., Nakamura, S., Sekiguchi, K., Sakuma, T., Yamamoto, T., Mori, T., Woltjen, K., Nakagawa, M., Yamamoto, T., Takahashi, K., Yamanaka, S., Saitou, M. . Robust in vitro induction of human germ cell fate from pluripotent stem cells. Cell stem cell, 17(2), 178-194 (2015).

- Nakagawa, M., Taniguchi, Y., Senda, S., Takizawa, N., Ichisaka, T., Asano, K., Morizane, A., Doi, D., Takahashi, J., Nishizawa, M., Yoshida, Y., Toyoda, T., Osafune, K., Sekiguchi, K., & Yamanaka, S. . A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells. Scientific reports, 4(3594), 1-7 (2014).

- Doi, D., Samata, B., Katsukawa, M., Kikuchi, T., Morizane, A., Ono, Y., Sekiguchi, K., Nakagawa, M., Parmar, M., Takahashi, J. . Isolation of human induced pluripotent stem cell-derived dopaminergic progenitors by cell sorting for successful transplantation. Stem cell reports, 2(3), 337-350 (2014).

- Takashima, Y., Guo, G., Loos, R., Nichols, J., Ficz, G., Krueger, F., Oxley, D., Santos, F., Clarke, J., Mansfield, W., Reik, W., Bertone, P., Smith, A. . Resetting transcription factor control circuitry toward ground-state pluripotency in human. Cell, 158(6), 1254-1269 (2014).

- Fukuta, M., Nakai, Y., Kirino, K., Nakagawa, M., Sekiguchi, K., Nagata, S., Matsumoto, Y., Yamamoto, T., Umeda, K., Heike, T., Okumura, N., Koizumi, N., Sato, T., Nakahata, T., Saito, M., Otsuka, T., Kinoshita, S., Ueno, M., Ikeya, M., Toguchida, J. . Derivation of mesenchymal stromal cells from pluripotent stem cells through a neural crest lineage using small molecule compounds with defined media. PloS one, 9(12), e112291 (2014).

- Burridge, P. W., Matsa, E., Shukla, P., Lin, Z. C., Churko, J. M., Ebert, A. D., Lan, F., Diecke, S., Huber, B., Mordwinkin, N. M., Plews, J. R., Abilez, O. J., Cui, B., Gold, J. D., & Wu, J. C. . Chemically defined generation of human cardiomyocytes. Nature methods, 11(8), 855-860 (2014).

- Miyazaki, T., Futaki, S., Suemori, H., Taniguchi, Y., Yamada, M., Kawasaki, M., Hayashi, M., Kumagai, H., Nakatsuji, N., Sekiguchi, K., & Kawase, E. . Laminin E8 fragment support efficient adhesion and expansion of dissociated human pluripotent stem cells. Nature communications, 3(1236), 1-10 (2012).

- Taniguchi, Y., Ido, H., Sanzen, N., Hayashi, M., Sato-Nishiuchi, R., Futaki, S., & Sekiguchi, K. . The C-terminal region of laminin β chains modulates the integrin binding affinities of laminins. Journal of Biological Chemistry, 284(12), 7820-7831 (2009).

- Ido, H., Nakamura, A., Kobayashi, R., Ito, S., Li, S., Futaki, S., & Sekiguchi, K. . The requirement of the glutamic acid residue at the third position from the carboxyl termini of the laminin γ chains in integrin binding by laminins. Journal of Biological Chemistry, 282(15), 11144-11154 (2007).


FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES

Manufactured by : 
Nippi, Inc.
1-1-1 Senju-Midoricho, Adachi-ku, Tokyo, Japan

Greater Cell Adhesion & Proliferation
In the study referenced above, the Laminin-511 E8 fragment and other cell culture substrates were compared for their strength in adhering to hES cells. The horizontal axis of the graph shows the concentration of cell culture substrate, and the vertical axis, the OD value (optical density at 570nm). This result shows that the Laminin-511 E8 fragment adheres to cells more strongly than its competitors.

In the same study cited above, Human ES cells were passaged on Laminin-511 E8 fragment and other cell culture substrates. Upon singly dispersing human ES cell colonies at the time of passage, it was confirmed that single cells adhere rapidly to Laminin-511 E8 and proliferate immediately.
Maintain and Culture Single ES/iPS Cells with Greater Propagation
Using the Laminin-511 E8 fragment as a cell culture matrix enables you to culture single ES/iPS cells, thereby reducing the time required to optimize culture methods and drastically enhancing efficiency of cell cultures.
Culture more ES/iPS cells using Laminin-511 E8 fragment
The graph above compares the numbers of ES/iPS cells cultured by the conventional method (colony) for 30 days with those cultured by Laminin-511 E8 fragment. The results confirm that the number of cells multiplied by at least 200 when Laminin-511 E8 fragment was used.
**For Research Use Only. Not For Use in Diagnostic Procedures**  

iMatrix-511 – Expressed by CHO-S Cell
- Format: Solution (175ug/vial @0.5mg/mL) Solvent: PBS(-)) 
- 175ug coats roughly 2-6 six well plates via conventional method.

Click here to learn more about ECM Pre-mix method.
-  Protocol & MSDS

* Cat# N-892011 : Size 350ug (175ug x 2 tubes)
---- List Price: $270.00  

* Cat# N-892012 : Size 1050ug (175ug x 6 tubes)  
---- List Price: $690.00  

* Cat# N-892013 : Trial size (175ug x 1 tube) - Only one purchase per lab 
----- List Price: $80.00 (Incl. US Domestic Shipping Fee)


iMatrix-511 SILK – Expressed by transgenic silkworm cocoon
- Format: Solution (175ug/vial @0.5mg/mL) Solvent: PBS(-)) 
- 175ug coats roughly 2-6 six well plates via conventional method.
- Similar spec as standard iMatrix-511 but more cost effective.

Click here to learn more about ECM Pre-mix method.
Protocol & MSDS

* Cat# N-892021: Size 1050ug (175ug x 6 tubes)
---- List Price: Please contact our customer service dept. 

* Cat# N-892022 : Trial size (175ug x 1 tube) - Only one purchase per lab 
----- List Price: $80.00 (Incl. US Domestic Shipping Fee)


Easy iMatrix-511 (Pre-diluted at a concentration of 0.25ug/cm²)
- Ready to Use
- 100mL = roughly 16 6-well plate

Click here to learn more about ECM Pre-mix method.
MSDS

iMatrix-511 (CHO-S Cell)
* Cat# N-892018 : Size 100mL
----- List Price: Please contact our customer service dept.

iMatrix-511 (Transgenic silkworm cocoon)
* Cat# N-892024 : Size 100mL
----- List Price: Please contact our customer service dept.

¹ Miyazaki et al., Nature Commun. 3; 1236 (2012)
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                                            Key Benefits:

  - Ideal for Xeno-Free, Animal-Free, Feeder-Free Cell Culture
  - Proven to provide superior adhesion of human ES and iPS cells¹
  - Enables the passaging of single cells
  - Compatible across a wide variety of cell types
  - Makes it easy to achieve extended cultures of hES/hiPS cells

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