Iwai North America Inc.

541 Taylor Way Suite# 4
 San Carlos, CA 94070
 Phone : (650) 486-1541
Fax : (650) 394-8638
 Open weekdays 9 AM-6 PM (PST)
.........................................................................................................................................................................................................................................................
iMatrix-511 is an innovative cell culture matrix compatible with a wide variety of cell types, and exceptionally well suited for pluripotent stem cells. This product is comprised of recombinant Laminin-511 E8 protein fragments which permit ES/iPS cells to be maintained in xeno-free culture conditions, enable the passaging of single cells, and provide greater adhesion than full-length Laminin, Vitronectin or Matrigel¹.
 



With the conventional method, ES/iPS cells die when dispersed into single cells, making it necessary to maintain a cell colony when passaging.
Laminin-511 is a major component of the basement membrane used as a scaffold for pluripotent stem cells (ES/iPS cells), as it binds to integrin on cell surfaces. However Laminin-511 is a large protein (800kDa) composed of three chains (α-, β-, and γ-) forming a supramolecular aggregate, making it difficult to produce recombinantly. In order to overcome this challenge, Laminin-511 proteins were fragmented to find the smallest integrin-binding component and the E8 fragment was identified as the most promising result. In fact, hES cells were found to adhere more strongly to the E8 fragment than to the full-length protein.

Matrix for Cell Culture
iMatrix-511
Fig. 1
Product Basics & Structure
Catalog# and Pricing
Greater Cell Adhesion & Proliferation
In the study referenced above, the Laminin-511 E8 fragment and other cell culture substrates were compared for their strength in adhering to hES cells. The horizontal axis of the graph shows the concentration of cell culture substrate, and the vertical axis, the OD value (optical density at 570nm). This result shows that the Laminin-511 E8 fragment adheres to cells more strongly than its competitors.

In the same study cited above, Human ES cells were passaged on Laminin-511 E8 fragment and other cell culture substrates. Upon singly dispersing human ES cell colonies at the time of passage, it was confirmed that single cells adhere rapidly to Laminin-511 E8 and proliferate immediately.
Maintain and Culture Single ES/iPS Cells with Greater Propagation
Using the Laminin-511 E8 fragment as a cell culture matrix enables you to culture single ES/iPS cells, thereby reducing the time required to optimize culture methods and drastically enhancing efficiency of cell cultures.
Culture more ES/iPS cells using Laminin-511 E8 fragment
The graph above compares the numbers of ES/iPS cells cultured by the conventional method (colony) for 30 days with those cultured by Laminin-511 E8 fragment. The results confirm that the number of cells multiplied by at least 200 when Laminin-511 E8 fragment was used.
**For Research Use Only. Not For Use in Diagnostic Procedures**  

iMatrix-511 – Expressed by CHO-S Cell
- Format: Solution (175ug/vial @0.5mg/mL) Solvent: PBS(-)) 
- 175ug coats roughly 2-6 six well plates via conventional method.

Click here to learn more about ECM Pre-mix method.
-  Protocol & MSDS

* Cat# N-892011 : Size 350ug (175ug x 2 tubes)
---- List Price: $270.00 => Promotional Price $216.00  

* Cat# N-892012 : Size 1050ug (175ug x 6 tubes)  
---- List Price: $690.00 =>  Promotional Price $552.00

* Cat# N-892013 : Trial size (175ug x 1 tube) - Only one purchase per lab 
----- List Price: $80.00 (Incl. Continental US Shipping Fee -- for all others 
please inquire)

iMatrix-511 SILK – Expressed by transgenic silkworm cocoon
- Format: Solution (175ug/vial @0.5mg/mL) Solvent: PBS(-)) 
- 175ug coats roughly 2-6 six well plates via conventional method.
- Similar spec as standard iMatrix-511 but more cost effective.

Click here to learn more about ECM Pre-mix method.
Protocol & MSDS

* Cat# N-892021: Size 1050ug (175ug x 6 tubes)
---- List Price: $340.00

* Cat# N-892022 : Trial size (175ug x 1 tube) - Only one purchase per lab 
----- List Price: $80.00 (Incl. Continental US Shipping Fee -- for all others 
please inquire)

Easy iMatrix-511 (Pre-diluted at a concentration of 1.6ug/ml)
- Ready to Use
- 100mL = roughly 16 6-well plate (amount to coat well surface = 0.25ug/cm²)
Click here to learn more about ECM Pre-mix method.
MSDS

Easy iMatrix-511 (CHO-S Cell)
* Cat# N-892018 : Size 100mL
----- List Price: $120.00

* Cat# N-892019 : Trial Size (20mL) - Only one purchase per lab 
----- List Price: $80.00 (Incl. Continental US Shipping Fee -- for all others 
please inquire)

Easy iMatrix-511 SILK (Transgenic silkworm cocoon)
* Cat# N-892024 : Size 100mL
----- List Price: $95.00

* Cat# N-892025 : Trial Size (20mL)- Only one purchase per lab 
----- List Price: $80.00 (Incl. Continental US Shipping Fee -- for all others 
please inquire)
¹ Miyazaki et al., Nature Commun. 3; 1236 (2012)
.....................................................
                                            Key Benefits:

  - Ideal for Xeno-Free, Animal-Free, Feeder-Free Cell Culture
  - Proven to provide superior adhesion of human ES and iPS cells¹
  - Enables the passaging of single cells
  - Compatible across a wide variety of cell types
  - Makes it easy to achieve extended cultures of hES/hiPS cells

.....................................................

References and Literature

Ayabe, H., Anada, T., Kamoya, T., Sato, T., Kimura, M., Yoshizawa, E., Kikuchi, Shunyuu., Ueno, Yasuharu., Sekine, keisuke., J. Gray Camp., Treutlein, B., Ferguson, Autumn., Suzuki, Osamu., Takede, Takanori.. Optimal Hypoxia Regulates Human iPSC-Derived Liver Bud Differentiation through Intercellular TGFB Signaling. Stem Cell Reports, 11, 1-11, (2018)

Musah, S., Dimitrakakis, N., Camacho, D. M., Church, G. M., Ingber, D. E.. Directed differentiation of human induced pluripotent stem cells into mature kidney podocytes and establishment of a lomerulus Chip. Nature protocols, 13(7), 1662, (2018)

Ishii, K., Sakurai, H., Suzuki, N., Mabuchi, Y., Sekiya, I., Sekiguchi, K., Akazawa, C.. Recapitulation of Extracellular LAMININ Environment Maintains Stemness of Satellite Cells In Vitro. Stem Cell Reports, 10, 1-15, (2018)

Ishida, K., Xu, H., Sasakawa, N., Lung, M. S. Y., Kudryashev, J. A., Gee, P., & Hotta, A.. Site-specific randomization of the endogenous genome by a regulatable CRISPR-Cas9 piggyBac system in human cells. Scientific Reports, 8(1), 310, (2018)

Takayama, K., Hagihara, Y., Toba, Y., Sekiguchi, K., Sakurai, F., Mizuguchi, H.. Enrichment of high-functioning human iPS cell-derived hepatocyte-like cells for pharmaceutical research. Biomaterials, (2018)

Akiyama, T., Sato, S., Chikazawa-Nohtomi, N., Soma, A., Kimura, H., Wakabayashi, S., Ko, S. B., Ko, M. S.. Efficient differentiation of human pluripotent stem cells into skeletal muscle cells by combining RNA-based MYOD1-expression and POU5F1-silencing. Scientific Reports, 8(1), 1189, (2018)

Saito, A., Ooki, A., Nakamura, T., Onodera, S., Hayashi, K., Hasegawa, D., Okudaira,T., Watanabe, K., Kato, H., Onda, T., Watanabe, A., Kosaki, K., Nishimura, K., Ohtaka, Manami., Nakanishi, M., Sakamoto, T., Yamaguchi, A., Sueishi, K., Azuma, T.. Targeted reversion of induced pluripotent stem cells from patients with human cleidocranial dysplasia improves bone regeneration in a rat calvarial bone defect model. Stem Cell Research & Therapy, 9(1), 12, (2018)

Yamauchi, K., Li, J., Morikawa, K., Liu, L., Shirayoshi, Y., Nakatsuji, N., Elliott, A. D., Hisatome, I., Suemori, H..Isolation and characterization of ventricular-like cells derived from NKX2-5 eGFP/w and MLC2v mCherry/w double knock-in human pluripotent stem cells. Biochemical and Biophysical Research Communications, 495(1), 1278-1284, (2018)

Mae, S., Ryosaka, M., Toyoda, T., Matsuse, K., Oshima, Y., Tsujimoto, H., Okumura, S., Shibasaki, A., Osafune, K.. Generation of branching ureteric bud tissues from human pluripotent stem cells. Biochemical and biophysical research communications, 495(1), 954-961, (2018)

Kagihiro, M., Fukumori, K., Aoki, T., Ungkulpasvich, U., Mizutani, M., Viravaidya-Pasuwat, K., & Kino-oka, M.. Kinetic analysis of cell decay during the filling process: Application to lot size determination in manufacturing systems for human induced pluripotent and mesenchymal stem cells. Biochemical Engineering Journal, 131, 31-38, (2018)

Zhang, R. R., Koido, M., Tadokoro, T., Ouchi, R., Matsuno, T., Ueno, Y., Sekine, K., Takebe, T., Taniguchi, H.. Human iPSC-Derived Posterior Gut Progenitors Are Expandable and Capable of Forming Gut and Liver Organoids. Stem Cell Reports, 10(2), 1?14, (2018)

Oshima, K., Saiki, N., Tanaka, M., Imamura, H., Niwa, A., Tanimura, A., Nagahashi, A., Hirayama, A., Okitac, K., Hotta, A., Kitayama, S., Osawa, M., Kaneko, S., Watanabe, A., Asaka, I., Fujibuchi, W., Imai, K., Yabe, H., Kamachi, Y., Hara, J., Kojima, S., Tomita, M., Soga, T., Noma, T., Nonoyama, S., Nakahata, T., Saito, MK.. Human AK2 links intracellular bioenergetic redistribution to the fate of hematopoietic progenitors. Biochemical and Biophysical Research Communications, 497(2), 719-725, (2018)

Sougawa, N., Miyagawa, S., Fukushima, S., Kawamura, A., Yokoyama, J., Ito, E., Harada, A., Okimoto, K., Mochisuki-Oda, N., Saito, A., Sawa, Y.. Immunologic targeting of CD30 eliminates tumourigenic human pluripotent stem cells, allowing safer clinical application of hiPSC-based cell therapy. Scientific Reports, 8(1), 3726, (2018)

Yasuda, S. Y., Ikeda, T., Shahsavarani, H., Yoshida, N., Nayer, B., Hino, M., Vartak-Sharma, N.,Suemori, H., Hasegawa, K.. Chemically defined and growth-factor-free culture system for the expansion and derivation of human pluripotent stem cells. Nature Biomedical Engineering, 2(3), 173, (2018)

Kim, S. I., Matsumoto, T., Kagawa, H., Nakamura, M., Hirohata, R., Ueno, A., Ohishi, M., Sakuma, T., Soga, T., Yamamoto, T., Woltjen, K.. Microhomology-assisted scarless genome editing in human iPSCs. Nature Communications, 9(1), 939, (2018)

Li, L., Roh, J. H., Chang, E. H., Lee, Y., Lee, S., Kim, M., Koh, W., Chang, J. W., Kim, H. J., Nakanishi, M., Barker, R. A., Na, D. L., Song, J.. iPSC Modeling of Presenilin1 Mutation in Alzheimer’s Disease with Cerebellar Ataxia. Experimental Neurobiology, 27, 27(5), 350-364, (2018)

Hayashi, R., Ishikawa, Y., Katori, R., Sasamoto, Y., Taniwaki, Y., Takayanagi, Tsujikawa, M., Sekiguchi, K., Quantock, A. J., Nishida, K. . Coordinated generation of multiple ocular-like cell lineages and fabrication of functional corneal epithelial cell sheets from human iPS cells. Nature Protocols, 12(4), 683-696, (2017)

Kikuchi, T., Morizane, A., Okita, K., Nakagawa, M., Yamakado, H., Inoue, H., Takahashi, R., Takahashi, J. . Idiopathic Parkinson’s disease patient‐derived induced pluripotent stem cells function as midbrain dopaminergic neurons in rodent brains. Journal of Neuroscience Research, 95(9),1829-37, (2017)

Miyazaki, T., Isobe, T., Nakatsuji, N., & Suemori, H. . Efficient Adhesion Culture of Human Pluripotent Stem Cells Using Laminin Fragments in an Uncoated Manner. Scientific Reports, 7(41165), 1-8, (2017)

Goparaju, S. K., Kohda, K., Ibata, K., Soma, A., Nakatake, Y., Akiyama, T., Wakabayashi, S., Matsushita, M., Sakota, M., Kimura, H., Yuzaki, M., Shigeru B. H. Ko & Minoru S. H. Ko. . Rapid differentiation of human pluripotent stem cells into functional neurons by mRNAs encoding transcription factors. Scientific Reports, 7, 42367, (2017)

Musah, S., Mammoto, A., Ferrante, C. T., Jeanty, S.S., Hirano-Kobayashi, M., Mammoto, T., Roberts, K., Chung, S., Novak, R., Ingram, M., Fatanat-Didar, T., Koshy, S., Weaver, C. J., Church, M. G., Ingber, F. D. . Mature induced-pluripotent-stem-cell-derived human podocytes reconstitute kidney glomerular-capillary-wall function on a chip. Nature Biomedical Engineering, 1 (0069), (2017)

Camp, J. G., Sekine, K., Gerber, T., Loeffler-Wirth, H., Binder, H., Gac, M., Kanton, S., Kageyama, J., Damm, G., Seehofer, D., Belicova, L., Barsacchi, M., Barsacchi, R., Okuda, R., Yoshizawa, E., Kimura, M., Ayabe, H., Taniguchi, H., Takebe, T., & Belicova, L.. Multilineage communication regulates human liver bud development from pluripotency. Nature, 546, 533-538, (2017)

Polisetti, N., Sorokin, L., Okumura, N., Koizumi, N., Kinoshita, S., Kruse, F. E., and Schlotzer-Schrehardt, U. Laminin-511 and-521-based matrices for efficient ex vivo-expansion of human limbal epithelial progenitor cells. Scientific Reports, 7, 5152, (2017)

Hongo, A., Okumura, N., Nakahara, M., Kay, E. P., & Koizumi, N.. The Effect of a p38 Mitogen-Activated Protein Kinase Inhibitor on Cellular Senescence of Cultivated Human Corneal Endothelial CellsEffect of a p38 MAPK Inhibitor on Corneal Endothelial Cells. Investigative Ophthalmology & Visual Science, 58(9), 3325-3334, (2017)

Taniguchi, Y., Li, S., Takizawa, M., Oonishi, E., Toga, J., Yagi, E., & Sekiguchi, K. Probing the acidic residue within the integrin binding site of laminin-511 that interacts with the metal ion-dependent adhesion site of α6β1 integrin. Biochemical and Biophysical Research Communications, 487(3), 525-531, (2017)

Sekine, S. I., Kondo, T., Murakami, N., Imamura, K., Enami, T., Shibukawa, R., Tsukita, K., Funayama, M., Inden, M., Kurita, H., Hozumi, I., Inoue, H.. Induced pluripotent stem cells derived from a patient with familial idiopathic basal ganglia calcification (IBGC) caused by a variant in SLC20A2 gene. Stem Cell Research, (2017)

Tan, G. W., Kondo, T., Murakami, N., Imamura, K., Enami, T., Tsukita, K., Shibukawa, R., Funayama, M., Matsumoto, R., Ikeda, I., Takahashi, R., Inoue, H.. Induced pluripotent stem cells derived from an autosomal dominant lateral temporal epilepsy (ADLTE) patient carrying S473L mutation in leucine-rich glioma inactivated 1 (LGI1). Stem Cell Research, (2017)

Sato-Nishiuchi, R., Li, S., Ebisu, F., Sekiguchi, K.. Recombinant laminin fragments endowed with collagen-binding activity: A tool for conferring laminin-like cell-adhesive activity to collagen matrices. Matrix Biology, (2017)

Kikuchi, T., Morizane, A., Doi, D., Magotani, H., Onoe, H., Hayashi, T., Mizuma, H., Takara, S., Takahashi, R., Inoue, H., Morita, S., Yamamoto, M., Okita, K., Nakagawa, M., Parmar, M., Takahashi, J.. Human iPS cell-derived dopaminergic neurons function in a primate Parkinson’s disease model. Nature, 548, 592-596, (2017)

Takizawa, M., Arimori, T., Taniguchi, Y., Kitago, Y., Yamashita, E., Takagi, J., Sekiguchi, K.. Mechanistic basis for the recognition of laminin-511 by α6β1 integrin. Science Advances, 3(9), e1701497, (2017)

Morizane, A., Kikuchi, T., Hayashi, T., Mizuma, H., Takara, S., Doi, H., Mawatari, A., Glasser, M. F., Shiina, T., Ishigaki, H., Itoh, Y., Okita, K., Yamasaki, E., Doi, D., Onoe, H., Ogasawara, K., Yamanaka, S., and Takahashi, J. . MHC matching improves engraftment of iPSC-derived neurons in non-human primates. Nature Communications, 8(1), 385, (2017)

Kikuchi, T., Morizane, A., Doi, D., Magotani, H., Onoe, H., Hayashi, T., Mizuma, H., Takara, S., Takahashi, R., Inoue, H., Morita, S., Yamamoto, M., Okita, K., Nakagawa, M., Parmar, M., Takahashi, J. . human ips cell-derived dopaminergic neurons function in a primate Parkinson’s disease model. Nature, 548(7669), 592-596, (2017)

Kojima, Y., Sasaki, K., Yokobayashi, S., Sakai, Y., Nakamura, T., Yabuta, Y., Nakaki, F., Nagaoka, S., Woltjen, K., Hotta, A., Yamamoto, T., Saitou, M.. Evolutionarily Distinctive Transcriptional and Signaling Programs Drive Human Germ Cell Lineage Specification from Pluripotent Stem Cells. Cell Stem Cell, 21(4), 517-532.e5, (2017)

Taguchi, A., & Nishinakamura, R.. Higher-Order Kidney Organogenesis from Pluripotent Stem Cells. Cell Stem Cell, 21. (2017)

Takebe, T., Sekine, K., Kimura, M., Yoshizawa, E., Ayano, S., Koido, M., Funayama, S., Nakanishi, N., Hisai, T., Kobayashi, T., Kasai, T., Kitada, R., Mori, A., Ayabe, H., Ejiri, Y., Amimoto, N., Yamazaki, Y., Ogawa, S., Ishikawa, M., Kiyota, Y., Sato, Y., Nozawa, K., Okamoto, S., Ueno, Y., Kasai, T.. Massive and Reproducible Production of Liver Buds Entirely from Human Pluripotent Stem Cells. Cell Reports, 21(10), 2661-2670, (2017)

Uchimura, T., Otomo, J., Sato, M., Sakurai, H.. A human iPS cell myogenic differentiation system permitting high-throughput drug screening. Stem cell research, 25, 98-106, (2017)

Sougawa, N., Miyagawa, S., Fukushima, S., Saito, A., Yokoyama, J., Kitahara, M., Harada, A., Sato-Nishiuchi, R., Sekiguchi, K., Sawa, Y.. Novel Stem Cell Niches Laminin 511 Promotes Functional Angiogenesis Through Enhanced Stem Cell Homing by Modulating” Stem Cell Beds” in the Failed Heart.Circulation, 136(1), A15587, (2017)


Samata, B., Doi, D., Nishimura, K., Kikuchi, T., Watanabe, A., Sakamoto, Y., Kakuta, J., Ono, Y., & Takahashi, J.. Purification of functional human ES and iPSC-derived midbrain dopaminergic progenitors using LRTM1. Nature Communications, 7(13097), 1-11, (2016)

Hayashi, R., Ishikawa, Y., Sasamoto, Y., Katori, R., Nomura, N., Ichikawa, T., Araki, S., Soma, T., Kawasaki, S., Sekiguchi, K., Tsujikawa, M., Nishida, K., & Quantock, A. J.. Co-ordinated ocular development from human iPS cells and recovery of corneal function. Nature, 531(7594), 376-380, (2016)

Matsuno, K., Mae, S. I., Okada, C., Nakamura, M., Watanabe, A., Toyoda, T., Uchida, E., Osafune, K.. Redefining definitive endoderm subtypes by robust induction of human induced pluripotent stem cells. Differentiation; research in biological diversity, (2016)

Nishimura, K., Doi, D., Samata, B., Murayama, S., Tahara, T., Onoe, H., & Takahashi, J.. Estradiol Facilitates Functional Integration of iPSC-Derived Dopaminergic Neurons into Striatal Neuronal Circuits via Activation of Integrin α5β1. Stem cell reports, 6(4), 511-524, (2016)

Takayama, K., Mitani, S., Nagamoto, Y., Sakurai, F., Tachibana, M., Taniguchi, Y., Sekiguchi, K., Mizuguchi, H.. Laminin 411 and 511 promote the cholangiocyte differentiation of human induced pluripotent stem cells. Biochemical and biophysical research communications, 474(1), 91-96, (2016)

Kawamura, T., Miyagawa, S., Fukushima, S., Maeda, A., Kashiyama, N., Kawamura, A., Miki, K., Okita, K., Yoshida, Y., Shiina, T., Ogasawara, K., Miyagawa, S., Toda, K., Okuyama, H., Sawa, Y.. Cardiomyocytes derived from MHC-homozygous induced pluripotent stem cells exhibit reduced allogeneic immunogenicity in MHC-matched non-human primates. Stem cell reports, 6(3), 312-320, (2016).

Tanigawa, S., Taguchi, A., Sharma, N., Perantoni, A. O., & Nishinakamura, R.. Selective in vitro propagation of nephron progenitors derived from embryos and pluripotent stem cells. Cell reports, 15(4), 801-813, (2016)


Okumura, N., Kakutani, K., Numata, R., Nakahara, M., Schlotzer-Schrehardt, U., Kruse, F., Kinoshita. K., Koizumi, N.. Laminin-511 and-521 Enable Efficient In Vitro Expansion of Human Corneal Endothelial CellsLaminin-511 and-521 Enable Expansion of HCECs. Investigative ophthalmology & visual science, 56(5), 2933-2942, (2015)

Sasaki, K., Yokobayashi, S., Nakamura, T., Okamoto, I., Yabuta, Y., Kurimoto, K., Ohta, H., Moritoki, Y., Iwatani, C., Tsuchiya, H., Nakamura, S., Sekiguchi, K., Sakuma, T., Yamamoto, T., Mori, T., Woltjen, K., Nakagawa, M., Yamamoto, T., Takahashi, K., Yamanaka, S., Saitou, M.. Robust in vitro induction of human germ cell fate from pluripotent stem cells. Cell stem cell, 17(2), 178-194, (2015)

Nakagawa, M., Taniguchi, Y., Senda, S., Takizawa, N., Ichisaka, T., Asano, K., Morizane, A., Doi, D., Takahashi, J., Nishizawa, M., Yoshida, Y., Toyoda, T., Osafune, K., Sekiguchi, K., & Yamanaka, S. . A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells. Scientific reports, 4(3594), 1-7, (2014)

Doi, D., Samata, B., Katsukawa, M., Kikuchi, T., Morizane, A., Ono, Y., Sekiguchi, K., Nakagawa, M., Parmar, M., Takahashi, J.. Isolation of human induced pluripotent stem cell-derived dopaminergic progenitors by cell sorting for successful transplantation. Stem cell reports, 2(3), 337-350, (2014)

Takashima, Y., Guo, G., Loos, R., Nichols, J., Ficz, G., Krueger, F., Oxley, D., Santos, F., Clarke, J., Mansfield, W., Reik, W., Bertone, P., Smith, A.. Resetting transcription factor control circuitry toward ground-state pluripotency in human. Cell, 158(6), 1254-1269, (2014)

Fukuta, M., Nakai, Y., Kirino, K., Nakagawa, M., Sekiguchi, K., Nagata, S., Matsumoto, Y., Yamamoto, T., Umeda, K., Heike, T., Okumura, N., Koizumi, N., Sato, T., Nakahata, T., Saito, M., Otsuka, T., Kinoshita, S., Ueno, M., Ikeya, M., Toguchida, J. . Derivation of mesenchymal stromal cells from pluripotent stem cells through a neural crest lineage using small molecule compounds with defined media. PloS one, 9(12), e112291, (2014)

Burridge, P. W., Matsa, E., Shukla, P., Lin, Z. C., Churko, J. M., Ebert, A. D., Lan, F., Diecke, S., Huber, B., Mordwinkin, N. M., Plews, J. R., Abilez, O. J., Cui, B., Gold, J. D., & Wu, J. C. . Chemically defined generation of human cardiomyocytes. Nature methods, 11(8), 855-860, (2014)

Miyazaki, T., Futaki, S., Suemori, H., Taniguchi, Y., Yamada, M., Kawasaki, M., Hayashi, M., Kumagai, H., Nakatsuji, N., Sekiguchi, K., & Kawase, E. . Laminin E8 fragment support efficient adhesion and expansion of dissociated human pluripotent stem cells. Nature communications, 3(1236), 1-10, (2012)

Taniguchi, Y., Ido, H., Sanzen, N., Hayashi, M., Sato-Nishiuchi, R., Futaki, S., & Sekiguchi, K. . The C-terminal region of laminin β chains modulates the integrin binding affinities of laminins. Journal of Biological Chemistry, 284(12), 7820-7831, (2009)

Ido, H., Nakamura, A., Kobayashi, R., Ito, S., Li, S., Futaki, S., & Sekiguchi, K. . The requirement of the glutamic acid residue at the third position from the carboxyl termini of the laminin γ chains in integrin binding by laminins. Journal of Biological Chemistry, 282(15), 11144-11154, (2007)

FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES

Manufactured by:
Nippi, Inc.
1-1-1 Senju-Midoricho, Adachi-ku, Tokyo, Japan