Iwai North America Inc.

541 Taylor Way Suite# 4
 San Carlos, CA 94070
 Phone : (650) 486-1541
Fax : (650) 394-8638
 Open weekdays 9 AM-6 PM (PST)
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EV-CATCH 
(Extracellular Vesicle Column for Affinity-Tethering CHromatography)
Isolate pure and intact extracellular vesicles (EV) from: 

            - Cell culture media
            - Serum
            - Urine
            - and many other kinds of samples 

 ...all at a higher yield than ultracentrifugation!  
Coming Soon!!
                                                                        Fig. 1 
Affinity purification of EVs using EV-CATCH from conditioned medium of MDA-MB-231-D3H2LN cells.

a)Silver staining (upper) and immune blotting of CD9 (lower) in purified fractions. EVs containing CD9
    were eluted in fraction #7.
b)Nanoparticle tracking analysis (NTA) of EV’s purified by ultracentrifugation (U.C.).
c)NTA of EVs in fraction# 7
Extracellular Vesicle Isolation & Purification Column from 
Theoria Science Co., Ltd.
                                                                    Fig. 2
Yield of affinity-purified EVs (MDA-MB-231-D3H2LN cells) measured by ExoScreen¹

d)ExoScreen¹ used for measuring CD9 EVs in purified fractions.
e)Yield of EVs by EV-Catch (affinity purification) and ultracentrifugation measured by ExoScreen¹.

1. Yoshioka et al., Nat. Commun., 2014 “Ultra-sensitive liquid biopsy of circulating extracellular vesicles using ExoScreen”

                                                                    Fig. 3
Uptake of EVs in Parental Cells  
Purified EVs were labeled with PKH26 and incubated with parental cells at 37˚C for 5h and visualized by confocal microscope. EVs purified by ultracentrifugation or by EV-Catch were equally taken up by cells.
                                                                Fig. 4
EV-Catch (Affinity purification) of EVs from human Serum.

a)ExoScreen for measuring CD9 of EVs in purified fractions.
b)Yield of EVs from human serum measured by ExoScreen.
c)Siliver staining of purified fractions.
d)Nanoparticle tracking analysis (NTA) of EVs purified by ultracentrifugation (U.C.).
e)NTA of EVs in fraction #6.

*Yield of EVs was excessively high due to the underestimation of total CD9 signal in serum.

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