Product

BIOMIMESYS® is a new generation of mimetic hydroscaffold for 3D cell culture. Available in a ready-to-use format, it enables the culture of cells under physiological conditions that are representative of the microenvironment found in whole tissues. The highly porous structure of BIOMIMESYS® allows cells to diffuse into the 3D matrix, where they can fix and begin to develop. The structure and conformation of cells cultured in BIOMIMESYS®, such as cancer cells that spontaneously form spheroids, strongly resemble those formed in vivo. Also, the functionality of the BIOMIMESYS® matrix is demonstrated by the expression of genes and proteins at levels that are again more similar to those found in vivo. Cells cultured in BIOMIMESYS® give results that are more physiologically relevant than those grown in 2D culture and are closer to those obtained in vivo.

Composed of Hyaluronic acid (HA) - a major component of the cell’s extracellular matrix (ECM) - the BIOMIMESYS®Oncology hydroscaffold for 3D hepatocyte cultures is an ideal study model because it is;

Highly porous for the simple extraction of RNA and proteins
Biodegradable for easy cell retrieval
Transparent for direct visualization of cells
Representative of the cell microenvironment in terms of cell-cell interactions, 
    cell-matrix interactions and the expression of proteins and genes


BIOMIMESYS® Oncology is made from HYALURONIC ACID (HA), a glycosaminoglycan. Hyaluronic acid is a major component of the extracellular matrix (ECM), and it plays an IMPORTANT ROLE in connective tissue.

The HA-based hydroscaffolds are formed by crosslinking HA and type I collagen with ADH to form reticulated chains.

High molecular weight Hyaluronic acid and type I collagen.

• BIODEGRADABLE product.

PHYSICOCHEMICAL FEATURES
>  Porosity: 150-200um
>  Rheology: Young’s modulus: E= 0.5 ± 0.15kPa
>  Swelling ratio = 75 ± 10g/g 


Ready to use

BIOMIMESYS® hydroscaffold technology  is EASY & READY TO USE. Upon receiving the vacuum sealed 96-well plate open it (under a hood) and add the cells directly on top of the matrix. Changing the culture medium is easy as well. To remove medium, simply draw the medium with a pipette between the matrix and the edge of the well. To refresh the medium, place fresh medium onto the surface of the matrix.

Compatible Technologies

BIOMIMESYS® hydroscaffold has many properties (transparent, porous, and biodegradable) that make it ideal for use with numerous downstream applications. The growing cells are easily retrieved from the matrix by a gentle and rapid procedure, meaning that cells cultured in BIOMIMESYS® may be analyzed using all technologies as shown below.

TRANSPARENT --- Microscopy, Plate Reader (OD, fluorescence, luminescence)
PORIOUS --- PCRsWestern-Blot, ELISA
BIODEGRADABLE --- Flow cytometry
SOLID --- Histology

Successfully Tested Cells

Cancer Cells Lines & Primary Tumors
Human brain metastasis: SA87
Human breast adenocarcinoma : MCF-7
Human breast carcinoma : CAL-51
Human cervix adenocarcinoma : HeLa
Human colorectal adenocarcinoma : DLD-1, HT29, Caco-2
Human glioblastoma : CB109 / CB74 / CB191
Human liver hepatocellular carcinoma : HepG2
Human liver hepatoma : PLC / PRF-5
Human lung carcinoma : NCI-H460
Human osteosarcoma : SaOs
Human ovarian carcinoma : IGROV-1
Human pancreas carcinoma : PANC-1
Human prostate cancer : PC3

* Proof of Concept
SPHEROID GROWTH, VIABILITY AND CELL PROLIFERATION
VISUALISATION OF EXTRACELLULAR MATRIX SECRETION IN CANCER CELLS



Publications

“DIRECT EFFECT OF BEVACIZUMAB ON GLIOBLASTOMA CELL LINES IN VITRO“. SIMON T., COQUEREL B., ET AL. (2014) NEUROMOLECULAR MED.; 16(4):752-71
“SURVIVAL OF CORD BLOOD HAEMATOPOIETIC STEM CELLS IN A HYALURONAN HYDROGEL FOR EX VIVO BIOMIMICRY”. DEMANGE E., KASSIM Y., ET AL. (2012) J TISSUE ENG REGEN MED 7(11):901-10
“ELASTIN-DERIVED PEPTIDES: MATRIKINES CRITICAL FOR GLIOBLASTOMA CELL AGGRESSIVENESS IN A 3-D SYSTEM”. COQUEREL B., POYER F., ET AL. (2009). GLIA 57(16): 1716-26.
“COLLAGENS, STROMAL CELL-DERIVED FACTOR-1ALPHA AND BASIC FIBROBLAST GROWTH FACTOR INCREASE CANCER CELL INVASIVENESS IN A HYALURONAN HYDROGEL”. DAVID L., DULONG V., ET AL. (2008). CELL PROLIF 41(2): 348-64
“HYALURONAN HYDROGEL: AN APPROPRIATE THREE-DIMENSIONAL MODEL FOR EVALUATION OF ANTICANCER DRUG SENSITIVITY.” DAVID L., DULONG V., ET AL. (2008). ACTA BIOMATER 4(2): 256-63
“RETICULATED HYALURONAN HYDROGELS: A MODEL FOR EXAMINING CANCER CELL INVASION IN 3D.” DAVID L., DULONG V., ET AL. (2004). MATRIX BIOL 23(3): 183-93




Pricing

BIOMIMESYS® Oncology is sold for RESEARCH USE ONLY.



Innovative hydroscaffolds for 3D Cell Culture to better mimic the tissue microenvironment and provide more predictive tools for drug development and metabolic studies.
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Iwai North America Inc.
541 Taylor Way Suite# 4
 San Carlos, CA 94070
 Phone : (650) 486-1541
Fax : (650) 394-8638
 Open weekdays 9 AM-6 PM (PST)
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