Product

BIOMIMESYS® Oncology is a new generation of mimetic hydroscaffold for 3D cell culture. Available in a ready-to-use format, it enables the culture of cells under physiological conditions that are representative of the microenvironment found in whole tissues. The highly porous structure of BIOMIMESYS® allows cells to diffuse into the 3D matrix, where they can fix and begin to develop. The structure and conformation of cells cultured in BIOMIMESYS®, such as cancer cells that spontaneously form spheroids, strongly resemble those formed in vivo. Also, the functionality of the BIOMIMESYS® matrix is demonstrated by the expression of genes and proteins at levels that are again more similar to those found in vivo. Cells cultured in BIOMIMESYS® give results that are more physiologically relevant than those grown in 2D culture and are closer to those obtained in vivo.

Composed of Hyaluronic acid (HA) - a major component of the cell’s extracellular matrix (ECM) - the BIOMIMESYS®Oncology hydroscaffold for 3D hepatocyte cultures is an ideal study model because it is;

Highly porous for the simple extraction of RNA and proteins
Biodegradable for easy cell retrieval
Transparent for direct visualization of cells
Representative of the cell microenvironment in terms of cell-cell interactions, 
    cell-matrix interactions and the expression of proteins and genes


• BIOMIMESYS® Oncology is made from HYALURONIC ACID (HA), a glycosaminoglycan. Hyaluronic acid is a major component of the extracellular matrix (ECM), and it plays an IMPORTANT ROLE in connective tissue.

• The HA-based hydroscaffolds are formed by crosslinking HA and type I collagen with ADH to form reticulated chains.

• High molecular weight Hyaluronic acid and type I collagen.

• BIODEGRADABLE product.

• PHYSICOCHEMICAL FEATURES
>  Porosity: 150-200um
>  Rheology: Young’s modulus: E= 0.5 ± 0.15kPa
>  Swelling ratio = 75 ± 10g/g 
Publications

• “DIRECT EFFECT OF BEVACIZUMAB ON GLIOBLASTOMA CELL LINES IN VITRO“. SIMON T., COQUEREL B., ET AL. (2014) NEUROMOLECULAR MED.; 16(4):752-71

• “SURVIVAL OF CORD BLOOD HAEMATOPOIETIC STEM CELLS IN A HYALURONAN HYDROGEL FOR EX VIVO BIOMIMICRY”. DEMANGE E., KASSIM Y., ET AL. (2012) J TISSUE ENG REGEN MED 7(11):901-10

• “ELASTIN-DERIVED PEPTIDES: MATRIKINES CRITICAL FOR GLIOBLASTOMA CELL AGGRESSIVENESS IN A 3-D SYSTEM”. COQUEREL B., POYER F., ET AL. (2009). GLIA 57(16): 1716-26.

• “COLLAGENS, STROMAL CELL-DERIVED FACTOR-1ALPHA AND BASIC FIBROBLAST GROWTH FACTOR INCREASE CANCER CELL INVASIVENESS IN A HYALURONAN HYDROGEL”. DAVID L., DULONG V., ET AL. (2008). CELL PROLIF 41(2): 348-64

• “HYALURONAN HYDROGEL: AN APPROPRIATE THREE-DIMENSIONAL MODEL FOR EVALUATION OF ANTICANCER DRUG SENSITIVITY.” DAVID L., DULONG V., ET AL. (2008). ACTA BIOMATER 4(2): 256-63

• “RETICULATED HYALURONAN HYDROGELS: A MODEL FOR EXAMINING CANCER CELL INVASION IN 3D.” DAVID L., DULONG V., ET AL. (2004). MATRIX BIOL 23(3): 183-93
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Iwai North America Inc.
541 Taylor Way Suite# 4
 San Carlos, CA 94070
 Phone : (650) 486-1541
Fax : (650) 394-8638
 Open weekdays 9 AM-6 PM (PST)
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BioMIMESYS® is an Organ specific Extracellular Matrix (ECM) formed by crosslinking reaction of hydrosoluble modified Hyaluronic Acid (HA) and other ECM components (collagens, fibronectin, etc.) with ADH (Adipic acid dihydrazide), to create a "Hydroscaffold" 3D cell culture system.
About BIOMIMESYS® Oncology
Ready to use

BIOMIMESYS® Liver is EASY & READY TO USE. Upon receiving the vacuum sealed 96-well plate open it (under a hood) and add the cells directly on top of the matrix. Changing the culture medium is easy as well. To remove medium, simply draw the medium with a pipette between the matrix and the edge of the well. To refresh the medium, place fresh medium onto the surface of the matrix.

Successfully Tested Cells

Cancer Cells Lines & Primary Tumors
Human brain metastasis: SA87
Human breast adenocarcinoma : MCF-7
Human breast carcinoma : CAL-51
Human cervix adenocarcinoma : HeLa
Human colorectal adenocarcinoma : DLD-1, HT29, Caco-2
Human glioblastoma : CB109 / CB74 / CB191
Human liver hepatocellular carcinoma : HepG2
Human liver hepatoma : PLC / PRF-5
Human lung carcinoma : NCI-H460
Human osteosarcoma : SaOs
Human ovarian carcinoma : IGROV-1
Human pancreas carcinoma : PANC-1
Human prostate cancer : PC3

* Proof of Concept
SPHEROID GROWTH, VIABILITY AND CELL PROLIFERATION
VISUALISATION OF EXTRACELLULAR MATRIX SECRETION IN CANCER CELLS
Compatible Technologies

BIOMIMESYS® hydroscaffold has many properties (transparent, porous & biodegradable) that make it ideal for use with numerous downstream applications. The growing cells are easily retrieved from the matrix by a gentle and rapid procedure, meaning that cells cultured in BIOMIMESYS® may be analyzed using all technologies as shown below.
TRANSPARENT --- Microscopy, Plate Reader (OD, fluorescence, luminescence)
PORIOUS --- PCRsWestern-Blot, ELISA
BIODEGRADABLE --- Flow cytometry
SOLID --- Histology
Ready to use

BIOMIMESYS® Liver is EASY & READY TO USE. Upon receiving the vacuum sealed 96-well plate open it (under a hood) and add the cells directly on top of the matrix. Changing the culture medium is easy as well. To remove medium, simply draw the medium with a pipette between the matrix and the edge of the well. To refresh the medium, place fresh medium onto the surface of the matrix.

Pricing

BIOMIMESYS®Liver is sold for RESEARCH USE ONLY.